3p13 region a possible location of a tumor suppressor ghne involved in uveal melanoma

M. B. Lasi, F. Roccella, N. De Felice, M. Roccella, R. Rota, P. Grarnmatico

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Abstract

Purpose Uveal melanoma shows monosomy of chromosome 3 as one of the most frequent rearrangements This result leads to the supposition of the involvement of a tumor suppressor gene located on chromosome 3 in the tumorigenesis of this tumor In order to contribute to the identification of the genes responsible for uveal melanoma, we present a case having a structural aberration involving chromosome 3 and 22 Methods A primary cell culture (UM-1) was obtained from a choroida! melanoma, historically characterized as a spindle cell type (dimensions 22 x 15 \10 mm) We utilized F10 medium additioned with 10% Fetal Calf Serum, 1% L- Glutamine. 2% Penicillin-Streptomycin; cytogenetic study was pei formed using both GTG and CBG banding techniques and FISH analysis. Results In ibis cell culture a t(3,22)(p13.:p11) was evidenced, as the only present chromosomal aberration Conclusions. In view of this result, and considering the rearrangement as a possible primary aberration, we would like to stress that the 3p13 breakpoint could be a candidate region for a tumor suppressor gene having an important role in the pathogenesis of this neoplasm.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
Publication statusPublished - 1997

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Chromosomes, Human, Pair 3
Tumor Suppressor Genes
Calcium Gluconate
Monosomy
Chromosomes, Human, Pair 22
Neoplasms
Primary Cell Culture
Streptomycin
Glutamine
Fluorescence In Situ Hybridization
Cytogenetics
Chromosome Aberrations
Penicillins
Melanoma
Carcinogenesis
Cell Culture Techniques
Serum
Genes
Uveal melanoma

ASJC Scopus subject areas

  • Ophthalmology

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3p13 region a possible location of a tumor suppressor ghne involved in uveal melanoma. / Lasi, M. B.; Roccella, F.; De Felice, N.; Roccella, M.; Rota, R.; Grarnmatico, P.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

Lasi, M. B. ; Roccella, F. ; De Felice, N. ; Roccella, M. ; Rota, R. ; Grarnmatico, P. / 3p13 region a possible location of a tumor suppressor ghne involved in uveal melanoma. In: Investigative Ophthalmology and Visual Science. 1997 ; Vol. 38, No. 4.
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AU - Lasi, M. B.

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AU - De Felice, N.

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AU - Rota, R.

AU - Grarnmatico, P.

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N2 - Purpose Uveal melanoma shows monosomy of chromosome 3 as one of the most frequent rearrangements This result leads to the supposition of the involvement of a tumor suppressor gene located on chromosome 3 in the tumorigenesis of this tumor In order to contribute to the identification of the genes responsible for uveal melanoma, we present a case having a structural aberration involving chromosome 3 and 22 Methods A primary cell culture (UM-1) was obtained from a choroida! melanoma, historically characterized as a spindle cell type (dimensions 22 x 15 \10 mm) We utilized F10 medium additioned with 10% Fetal Calf Serum, 1% L- Glutamine. 2% Penicillin-Streptomycin; cytogenetic study was pei formed using both GTG and CBG banding techniques and FISH analysis. Results In ibis cell culture a t(3,22)(p13.:p11) was evidenced, as the only present chromosomal aberration Conclusions. In view of this result, and considering the rearrangement as a possible primary aberration, we would like to stress that the 3p13 breakpoint could be a candidate region for a tumor suppressor gene having an important role in the pathogenesis of this neoplasm.

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