\4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma

L. Cimino, D. Perrotti, R. Falcioni, S. J. Kennel, A. Sacchi

Research output: Contribution to journalArticle

Abstract

The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalCytotechnology
Volume5
Issue number1 Supplement
DOIs
Publication statusPublished - Feb 1991

Fingerprint

Lewis Lung Carcinoma
Integrins
Epitopes
Messenger RNA
Monoclonal antibodies
Monoclonal Antibodies
Northern Blotting
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology

Cite this

\4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma. / Cimino, L.; Perrotti, D.; Falcioni, R.; Kennel, S. J.; Sacchi, A.

In: Cytotechnology, Vol. 5, No. 1 Supplement, 02.1991, p. 45-48.

Research output: Contribution to journalArticle

Cimino, L. ; Perrotti, D. ; Falcioni, R. ; Kennel, S. J. ; Sacchi, A. / \4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma. In: Cytotechnology. 1991 ; Vol. 5, No. 1 Supplement. pp. 45-48.
@article{40b8fa349a2c4d67b82652100eb30e81,
title = "\4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma",
abstract = "The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.",
author = "L. Cimino and D. Perrotti and R. Falcioni and Kennel, {S. J.} and A. Sacchi",
year = "1991",
month = "2",
doi = "10.1007/BF00736805",
language = "English",
volume = "5",
pages = "45--48",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "1 Supplement",

}

TY - JOUR

T1 - \4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma

AU - Cimino, L.

AU - Perrotti, D.

AU - Falcioni, R.

AU - Kennel, S. J.

AU - Sacchi, A.

PY - 1991/2

Y1 - 1991/2

N2 - The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.

AB - The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.

UR - http://www.scopus.com/inward/record.url?scp=34249926811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249926811&partnerID=8YFLogxK

U2 - 10.1007/BF00736805

DO - 10.1007/BF00736805

M3 - Article

C2 - 22358951

AN - SCOPUS:34249926811

VL - 5

SP - 45

EP - 48

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 1 Supplement

ER -