Abstract
The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.
| Original language | English |
|---|---|
| Pages (from-to) | 45-48 |
| Number of pages | 4 |
| Journal | Cytotechnology |
| Volume | 5 |
| Issue number | 1 Supplement |
| DOIs | |
| Publication status | Published - Feb 1991 |
Fingerprint
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Biotechnology
Cite this
\4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma. / Cimino, L.; Perrotti, D.; Falcioni, R.; Kennel, S. J.; Sacchi, A.
In: Cytotechnology, Vol. 5, No. 1 Supplement, 02.1991, p. 45-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - \4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma
AU - Cimino, L.
AU - Perrotti, D.
AU - Falcioni, R.
AU - Kennel, S. J.
AU - Sacchi, A.
PY - 1991/2
Y1 - 1991/2
N2 - The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.
AB - The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.
UR - http://www.scopus.com/inward/record.url?scp=34249926811&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249926811&partnerID=8YFLogxK
U2 - 10.1007/BF00736805
DO - 10.1007/BF00736805
M3 - Article
C2 - 22358951
AN - SCOPUS:34249926811
VL - 5
SP - 45
EP - 48
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 1 Supplement
ER -