433-443 Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis

L. Van Bon, A. J. Affandi, J. Broen, R. B. Christmann, R. J. Marijnissen, L. Stawski, G. A. Farina, G. Stifano, A. L. Mathes, M. Cossu, M. York, C. Collins, M. Wenink, R. Huijbens, R. Hesselstrand, T. Saxne, M. DiMarzio, D. Wuttge, S. K. Agarwal, J. D. ReveilleS. Assassi, M. Mayes, Y. Deng, J. P H Drenth, J. De Graaf, M. Den Heijer, C. G M Kallenberg, M. Bijl, A. Loof, W. B. Van Den Berg, L. A B Joosten, V. Smith, F. De Keyser, R. Scorza, C. Lunardi, P. L C M Van Riel, M. Vonk, W. Van Heerde, S. Meller, B. Homey, L. Beretta, M. Roest, M. Trojanowska, R. Lafyatis, T. R D J Radstake

Research output: Contribution to journalArticle

Abstract

Background: Plasmacytoid dendritic cells have been implicated in the pathogenesis of systemic sclerosis through mechanisms beyond the previously suggested production of type I interferon. Methods: We isolated plasmacytoid dendritic cells from healthy persons and from patients with systemic sclerosis who had distinct clinical phenotypes. We then performed proteome-wide analysis and validated these observations in five large cohorts of patients with systemic sclerosis. Next, we compared the results with those in patients with systemic lupus erythematosus, ankylosing spondylitis, and hepatic fibrosis. We correlated plasma levels of CXCL4 protein with features of systemic sclerosis and studied the direct effects of CXCL4 in vitro and in vivo. Results: Proteome-wide analysis and validation showed that CXCL4 is the predominant protein secreted by plasmacytoid dendritic cells in systemic sclerosis, both in circulation and in skin. The mean (±SD) level of CXCL4 in patients with systemic sclerosis was 25,624±2652 pg per milliliter, which was significantly higher than the level in controls (92.5±77.9 pg per milliliter) and than the level in patients with systemic lupus erythematosus (1346±1011 pg per milliliter), ankylosing spondylitis (1368±1162 pg per milliliter), or liver fibrosis (1668±1263 pg per milliliter). CXCL4 levels correlated with skin and lung fibrosis and with pulmonary arterial hypertension. Among chemokines, only CXCL4 predicted the risk and progression of systemic sclerosis. In vitro, CXCL4 downregulated expression of transcription factor FLI1, induced markers of endothelial-cell activation, and potentiated responses of toll-like receptors. In vivo, CXCL4 induced the influx of inflammatory cells and skin transcriptome changes, as in systemic sclerosis. Conclusions: Levels of CXCL4 were elevated in patients with systemic sclerosis and correlated with the presence and progression of complications, such as lung fibrosis and pulmonary arterial hypertension.

Original languageEnglish
Pages (from-to)433-443
Number of pages11
JournalNew England Journal of Medicine
Volume370
Issue number5
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Medicine(all)

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    Van Bon, L., Affandi, A. J., Broen, J., Christmann, R. B., Marijnissen, R. J., Stawski, L., Farina, G. A., Stifano, G., Mathes, A. L., Cossu, M., York, M., Collins, C., Wenink, M., Huijbens, R., Hesselstrand, R., Saxne, T., DiMarzio, M., Wuttge, D., Agarwal, S. K., ... Radstake, T. R. D. J. (2014). 433-443 Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis. New England Journal of Medicine, 370(5), 433-443. https://doi.org/10.1056/NEJMoa1114576