5-Aminoisoquinolin-1(2H)-one, a water-soluble poly (ADP-ribose) polymerase (PARP) inhibitor reduces the evolution of experimental periodontitis in rats

Rosanna Di Paola, Emanuela Mazzon, Carmelo Muià, Dino Terrana, Salvatore Greco, Domenico Britti, Domenico Santori, Giacomo Oteri, Giancarlo Cordasco, Salvatore Cuzzocrea

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme activated by strand breaks in DNA, plays an important role in the tissue injury associated with ischaemia-reperfusion and inflammation. Recent studies have demonstrated that PARP activation plays a crucial role in the pathogenesis of acute periodontal injury. Aim: We have investigated the effect of 5-aminoisoquinolin-1(2H)-one (5-AIQ), a water-soluble PARP inhibitor, in a rat model of periodontitis. Materials and Methods: Periodontitis was induced in rats by placing a 2/0 braided silk ligature around the lower left first molar. At day eight, the gingivomucosal tissue encircling the mandibular first molar was removed for biochemical and histological analysis. Results and Conclusions: Ligation significantly induced an increased neutrophil infiltration and a positive staining for PARP activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Intraperitonial injection of 5-aminoisoquinolin-1(2H)-one (5-AIQ) (5 mg/kg daily for eight days) significantly decreased all of the parameters of inflammation as described above. This suggests that inhibition of PARP may represent a novel approach for the treatment of periodontal disease.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalJournal of Clinical Periodontology
Volume34
Issue number2
DOIs
Publication statusPublished - Feb 2007

Keywords

  • Alveolar bone loss
  • Neutrophil infiltration
  • PARP inhibitor
  • Periodontal diseases
  • Poly (ADP-ribose) polymerase (PARP)

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of '5-Aminoisoquinolin-1(2H)-one, a water-soluble poly (ADP-ribose) polymerase (PARP) inhibitor reduces the evolution of experimental periodontitis in rats'. Together they form a unique fingerprint.

Cite this