5-Lipoxygenase and cyclooxygenase-2 in porcine parasitic bronchopneumonia: Lmmunohistochemical and biochemical investigations

G. Marruchella, R. Giacominelli-Stuffler, M. Baffoni, M. Maccarrone

Research output: Contribution to journalArticle

Abstract

Eicosanoids are products of arachidonic acid metabolism and have numerous biological roles. The present study aimed to investigate the role of 5-lipoxygenase (5-LOX)- and cyclooxygenase-2 (COX-2)- dependent enzymatic pathways in the pathogenesis of porcine parasitic bronchopneumonia caused by Metastrongylus spp. Pulmonary tissue samples from healthy control and parasitized pigs were processed for histopathological, immunohistochemical and biochemical investigations. In control animals, immunohistochemistry demonstrated that 5-LOX and COX-2 expression was almost exclusively limited to the bronchiolar epithelial cells. Parasitized pigs had greater 5-LOX- and COX-2- specific immunoreactivity, involving a wide range of cell types within foci of granulomatous and eosinophilic bronchopneumonia. Biochemical investigations demonstrated the presence of 5-LOX (and the related product Leukotriene B4) and COX-2 (and the related product prostaglandin E2; PGE2) in all tissues under study. COX-2 activity and PGE2 concentration were significantly higher in diseased lungs compared with normal healthy controls. These findings demonstrate that 5-LOX and COX-2 are differentially expressed in normal versus lungworm-infected lungs and therefore suggest that both biochemical pathways are likely to be involved in the pathogenesis of porcine parasitic bronchopneumonia.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalJournal of Comparative Pathology
Volume142
Issue number2-3
DOIs
Publication statusPublished - Feb 2010

Keywords

  • Bronchopneumonia
  • Cyclooxygenase
  • Eicosanoids
  • Lipoxygenase
  • Lungworm
  • Pig

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • veterinary(all)

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