Abstract
Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 195-200 |
| Number of pages | 6 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 867 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 22 1986 |
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Keywords
- Chromatin organization
- DNA methylation
- HaeIII restriction endonuclease (EC 3.1.23.17)
- Nucleosome subpopulation
- proteinase K (EC 3.4.21.14)
- RNAase (EC 3.1.27.5)
- Staphylococcus aureus nuclease (EC 3.1.31.1)
- Transcriptional activity
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
Cite this
5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression. / Caiafa, Paola; Attinǎ, Marina; Cacace, Fulvio; Tomassetti, Antonella; Strom, Roberto.
In: BBA - Gene Structure and Expression, Vol. 867, No. 4, 22.08.1986, p. 195-200.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression
AU - Caiafa, Paola
AU - Attinǎ, Marina
AU - Cacace, Fulvio
AU - Tomassetti, Antonella
AU - Strom, Roberto
PY - 1986/8/22
Y1 - 1986/8/22
N2 - Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.
AB - Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.
KW - Chromatin organization
KW - DNA methylation
KW - HaeIII restriction endonuclease (EC 3.1.23.17)
KW - Nucleosome subpopulation
KW - proteinase K (EC 3.4.21.14)
KW - RNAase (EC 3.1.27.5)
KW - Staphylococcus aureus nuclease (EC 3.1.31.1)
KW - Transcriptional activity
UR - http://www.scopus.com/inward/record.url?scp=0022912458&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022912458&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(86)90034-5
DO - 10.1016/0167-4781(86)90034-5
M3 - Article
C2 - 3741873
AN - SCOPUS:0022912458
VL - 867
SP - 195
EP - 200
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 4
ER -