5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression

Paola Caiafa; Marina Attinǎ; Fulvio Cacace; Antonella Tomassetti; Roberto Strom

doi:10.1016/0167-4781(86)90034-5

5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression

Paola Caiafa, Marina Attinǎ, Fulvio Cacace, Antonella Tomassetti, Roberto Strom

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	BBA - Gene Structure and Expression
Volume	867
Issue number	4
DOIs	https://doi.org/10.1016/0167-4781(86)90034-5
Publication status	Published - Aug 22 1986

Keywords

Chromatin organization
DNA methylation
HaeIII restriction endonuclease (EC 3.1.23.17)
Nucleosome subpopulation
proteinase K (EC 3.4.21.14)
RNAase (EC 3.1.27.5)
Staphylococcus aureus nuclease (EC 3.1.31.1)
Transcriptional activity

ASJC Scopus subject areas

Biochemistry
Biophysics
Genetics
Structural Biology

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10.1016/0167-4781(86)90034-5

Cite this

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title = "5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression",

abstract = "Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.",

keywords = "Chromatin organization, DNA methylation, HaeIII restriction endonuclease (EC 3.1.23.17), Nucleosome subpopulation, proteinase K (EC 3.4.21.14), RNAase (EC 3.1.27.5), Staphylococcus aureus nuclease (EC 3.1.31.1), Transcriptional activity",

author = "Paola Caiafa and Marina Attinǎ and Fulvio Cacace and Antonella Tomassetti and Roberto Strom",

year = "1986",

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doi = "10.1016/0167-4781(86)90034-5",

language = "English",

volume = "867",

pages = "195--200",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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T1 - 5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression

AU - Caiafa, Paola

AU - Attinǎ, Marina

AU - Cacace, Fulvio

AU - Tomassetti, Antonella

AU - Strom, Roberto

PY - 1986/8/22

Y1 - 1986/8/22

N2 - Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.

AB - Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.

KW - Chromatin organization

KW - DNA methylation

KW - HaeIII restriction endonuclease (EC 3.1.23.17)

KW - Nucleosome subpopulation

KW - proteinase K (EC 3.4.21.14)

KW - RNAase (EC 3.1.27.5)

KW - Staphylococcus aureus nuclease (EC 3.1.31.1)

KW - Transcriptional activity

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U2 - 10.1016/0167-4781(86)90034-5

DO - 10.1016/0167-4781(86)90034-5

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AN - SCOPUS:0022912458

VL - 867

SP - 195

EP - 200

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 4

ER -

5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression

Abstract

Keywords

ASJC Scopus subject areas

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