5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression

Paola Caiafa, Marina Attinǎ, Fulvio Cacace, Antonella Tomassetti, Roberto Strom

Research output: Contribution to journalArticle

Abstract

Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalBBA - Gene Structure and Expression
Volume867
Issue number4
DOIs
Publication statusPublished - Aug 22 1986

Fingerprint

5-Methylcytosine
Nucleosomes
Gene expression
Gene Expression
High Mobility Group Proteins
Density Gradient Centrifugation
Centrifugation
DNA-Directed RNA Polymerases
Ionic strength
Edetic Acid
Gas chromatography
Histones
Osmolar Concentration
Gas Chromatography-Mass Spectrometry
Chromatin
Mass spectrometry
Sucrose
DNA

Keywords

  • Chromatin organization
  • DNA methylation
  • HaeIII restriction endonuclease (EC 3.1.23.17)
  • Nucleosome subpopulation
  • proteinase K (EC 3.4.21.14)
  • RNAase (EC 3.1.27.5)
  • Staphylococcus aureus nuclease (EC 3.1.31.1)
  • Transcriptional activity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression. / Caiafa, Paola; Attinǎ, Marina; Cacace, Fulvio; Tomassetti, Antonella; Strom, Roberto.

In: BBA - Gene Structure and Expression, Vol. 867, No. 4, 22.08.1986, p. 195-200.

Research output: Contribution to journalArticle

Caiafa, Paola ; Attinǎ, Marina ; Cacace, Fulvio ; Tomassetti, Antonella ; Strom, Roberto. / 5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression. In: BBA - Gene Structure and Expression. 1986 ; Vol. 867, No. 4. pp. 195-200.
@article{fca806af285344209c4273e6ea6f62cc,
title = "5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression",
abstract = "Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.",
keywords = "Chromatin organization, DNA methylation, HaeIII restriction endonuclease (EC 3.1.23.17), Nucleosome subpopulation, proteinase K (EC 3.4.21.14), RNAase (EC 3.1.27.5), Staphylococcus aureus nuclease (EC 3.1.31.1), Transcriptional activity",
author = "Paola Caiafa and Marina Attinǎ and Fulvio Cacace and Antonella Tomassetti and Roberto Strom",
year = "1986",
month = "8",
day = "22",
doi = "10.1016/0167-4781(86)90034-5",
language = "English",
volume = "867",
pages = "195--200",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - 5-Methylcytosine levels in nucleosome subpopulations differently involved in gene expression

AU - Caiafa, Paola

AU - Attinǎ, Marina

AU - Cacace, Fulvio

AU - Tomassetti, Antonella

AU - Strom, Roberto

PY - 1986/8/22

Y1 - 1986/8/22

N2 - Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.

AB - Sucrose density gradient centrifugation in the presence or absence of Na-EDTA and at different ionic strengths allows one to obtain well-defined nucleosome subpopulations the DNA of which, examined by gas chromatography-mass spectrometry, is in all cases hypermethylated as compared to spacer regions, but to a different extent for the different subpopulations. The various nucleosomes differ also in their content of histones and of high-mobility-group proteins, as well as in the levels of RNA polymerase activity associated with them. Such data suggest that these nucleosome subpopulations originate from chromatin fractions differently involved in gene expression.

KW - Chromatin organization

KW - DNA methylation

KW - HaeIII restriction endonuclease (EC 3.1.23.17)

KW - Nucleosome subpopulation

KW - proteinase K (EC 3.4.21.14)

KW - RNAase (EC 3.1.27.5)

KW - Staphylococcus aureus nuclease (EC 3.1.31.1)

KW - Transcriptional activity

UR - http://www.scopus.com/inward/record.url?scp=0022912458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022912458&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(86)90034-5

DO - 10.1016/0167-4781(86)90034-5

M3 - Article

C2 - 3741873

AN - SCOPUS:0022912458

VL - 867

SP - 195

EP - 200

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 4

ER -