Aβ1-42 Detection in CSF of Alzheimer's disease is influenced by temperature: Indication of reversible Aβ1-42 aggregation?

Giulia M. Sancesario, Zaira Esposito, Marzia Nuccetelli, Sergio Bernardini, Roberto Sorge, Alessandro Martorana, Giorgio Federici, Giorgio Bernardi, Giuseppe Sancesario

Research output: Contribution to journalArticlepeer-review

Abstract

Amyloid-beta 1-42 (Aβ1-42), peptide detectable in cerebrospinal fluid (CSF), has been extensively studied as diagnostic marker for Alzheimer's disease; however, results are variable. We investigated whether Aβ1-42 detection in CSF may be affected by handling temperature after lumbar puncture. CSF was collected from patients affected by probable AD (n=27), other dementias (OD) (n=24), or other neurological disorders without cognitive impairment (OND) (n=23). After lumbar puncture, CSF samples were either maintained at 37 °C, or handled according to standard procedures and centrifuged at 4 °C for 10 min; thereafter, one aliquot was further stored at 4 °C and another at 37 °C, before freezing all samples 90 min later at - 80 °C, pending analysis. Aβ1-42 and total tau were determined using a commercially available sandwich enzyme-linked immunosorbent assay ELISA. Reduced Aβ1-42 and increased total tau CSF levels were confirmed as characteristic hallmarks of the OD and AD groups, providing standard measurement in samples stored at 4 °C before freezing. However, avoiding cooling or reheating CSF from 4 to 37 °C before freezing strikingly increased the Aβ1-42 concentration detectable in the AD group (P

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalExperimental Neurology
Volume223
Issue number2
DOIs
Publication statusPublished - Jun 2010

Keywords

  • Alzheimer's disease
  • Biological marker
  • Cerebrospinal fluid
  • Dementia
  • Oligomers

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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