A 50 Hz sinusoidal magnetic field does not damage MG-63 three-dimensional tumor spheroids but induces changes in their invasive properties

Maria Teresa Santini, Gabriella Rainaldi, Antonella Ferrante, Paola Indovina, Gianfranco Donelli, Pietro Luigi Indovina

Research output: Contribution to journalArticlepeer-review

Abstract

The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 1 mT can damage MG-63 osteosarcoma spheroids and induce variations in the invasive properties of these three-dimensional model systems after 2 days of exposure was investigated. Specifically, possible damage induced by these fields was examined by determining changes in spheroid surface morphology (light microscopy), growth (spheroid diameter and protein content determination), lactate dehydrogenase release, and reduced glutathione amount. Possible changes in the invasive properties were studied by invasion chambers. The results show no induction of cell damage by ELF fields while invasion chamber assays demonstrate a significant increase in the invasive potential of exposed spheroids. In order to determine if the fibronectin or hyaluronan receptors are involved, Western blot analysis was conducted on these two proteins. No significant variations were observed in either receptor in MG-63 multicellular tumor spheroids.

Original languageEnglish
Pages (from-to)132-141
Number of pages10
JournalBioelectromagnetics
Volume27
Issue number2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • ELF
  • Fibronectin receptor
  • Hyaluronan receptor
  • Osteosarcoma cells
  • Tumor cell invasion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics

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