A 6-hour microneutralization assay for human cytomegalovirus antibody by using monoclonal antibodies

Giuseppe Gerna, M. Grazia Revello, Elena Percivalle, Maria Torsellini

Research output: Contribution to journalArticlepeer-review

Abstract

Human cytomegalovirus (HCMV) was quantified at 6, 24, and 96 h post infection by counting the number of infected cells which were stained by the immunoperoxidase technique using monoclonal antibodies reactive to different HCMV non-structural and structural proteins. A linear dose-effect relationship was found at the three indicated times. Three rapid neutralization (Nt) assays (one for each time) were developed, using the same virus inoculum. Human sera from individuals with primary, reactivated or remote HCMV infection gave comparable Nt antibody titers with the three assays. The 6-hour rapid Nt assay appears highly suitable for determination of HCMV immune status and provides results the same day of blood collection.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalSerodiagnosis and Immunotherapy in Infectious Disease
Volume4
Issue number3
DOIs
Publication statusPublished - 1990

Keywords

  • human cytomegalovirus antibody
  • microneutralization
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)

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