A 700 MHz 1H-NMR study reveals apoptosis-like behavior in human K562 erythroleukemic cells exposed to a 50 Hz sinusoidal magnetic field

M. T. Santini, A. Ferrante, R. Romano, G. Rainaldi, A. Motta, G. Donelli, P. Vecchia, P. L. Indovina

Research output: Contribution to journalArticle

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Abstract

Purpose: To study cell damage and possible apoptosis in K562 human erythroleukemic cells exposed for 2 h to an extremely low frequency (ELF) 50 Hz sinusoidal magnetic field with a magnetic induction of either 1 or 5 mT using high resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. Materials and methods: One-dimensional 1H-NMR spectra were obtained on whole K562 cells and perchloric acid extracts of these cells. In addition, two-dimensional 1H-NMR spectra were also acquired. Cell damage was examined by lactate dehydrogenase release and changes in cell growth were monitored by growth curve analyses, bromodeoxyuridine incorporation and Ki67 antigen localization. Cell death (necrosis and apoptosis) were also studied by using the chromatin dye Hoechst 33258. Results: The variations in numerous metabolites observed with 1H-NMR reveal apoptosis-like behavior in response of K562 cells to ELF fields. Conclusion: 1H-NMR can be extremely useful in studying the effects of ELF fields on cells. In particular, the variations in metabolites which suggest apoptosis-like behavior occur when the cells are not identifiable as apoptotic by more traditional techniques.

Original languageEnglish
Pages (from-to)97-113
Number of pages17
JournalInternational Journal of Radiation Biology
Volume81
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

K562 Cells
apoptosis
human behavior
Cell death
Magnetic Fields
magnetic fields
Nuclear magnetic resonance
Apoptosis
Magnetic fields
extremely low frequencies
nuclear magnetic resonance
cells
metabolites
Metabolites
Magnetic Resonance Spectroscopy
Cells
Ki-67 Antigen
damage
Bisbenzimidazole
nuclear magnetic resonance spectroscopy

Keywords

  • 50 Hz sinusoidal magnetic field
  • H-NMR
  • Apoptosis
  • K562 erythroleukemic cell

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

A 700 MHz 1H-NMR study reveals apoptosis-like behavior in human K562 erythroleukemic cells exposed to a 50 Hz sinusoidal magnetic field. / Santini, M. T.; Ferrante, A.; Romano, R.; Rainaldi, G.; Motta, A.; Donelli, G.; Vecchia, P.; Indovina, P. L.

In: International Journal of Radiation Biology, Vol. 81, No. 2, 02.2005, p. 97-113.

Research output: Contribution to journalArticle

Santini, M. T. ; Ferrante, A. ; Romano, R. ; Rainaldi, G. ; Motta, A. ; Donelli, G. ; Vecchia, P. ; Indovina, P. L. / A 700 MHz 1H-NMR study reveals apoptosis-like behavior in human K562 erythroleukemic cells exposed to a 50 Hz sinusoidal magnetic field. In: International Journal of Radiation Biology. 2005 ; Vol. 81, No. 2. pp. 97-113.
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