TY - JOUR
T1 - A bioactive olive pomace extract prevents the death of murine cortical neurons triggered by NMDAR over-activation
AU - Franchi, Alice
AU - Pedrazzi, Marco
AU - Casazza, Alessandro Alberto
AU - Enrico, Millo
AU - Damonte, Gianluca
AU - Salis, Annalisa
AU - Liessi, Nara
AU - Onofri, Franco
AU - Marte, Antonella
AU - Casagrande, Silvia
AU - Roberta, De Tullio
AU - Perego, Patrizia
AU - Averna, Monica
N1 - Funding Information:
Funding: This work was supported by the grant FRA2017 from University of Genoa to A.M. and by the grant FRA2018 from University of Genoa to D.T.R.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - We have recently demonstrated that bioactive molecules, extracted by high pressure and temperature from olive pomace, counteract calcium-induced cell damage to different cell lines. Here, our aim was to study the effect of the same extract on murine cortical neurons, since the preservation of the intracellular Ca2+-homeostasis is essential for neuronal function and survival. Accordingly, we treated neurons with different stimuli in order to evoke cytotoxic glutamatergic activation. In these conditions, the high-pressure and temperature extract from olive pomace (HPTOPE) only abolished the effects of N-methyl-d-aspartate (NMDA). Particularly, we observed that HPTOPE was able to promote the neuron rescue from NMDA-induced cell death. Moreover, we demonstrated that HPTOPE is endowed with the ability to maintain the intracellular Ca2+-homeostasis following NMDA receptor overactivation, protecting neurons from Ca2+-induced adverse effects, including aberrant calpain proteolytic activity. Moreover, we highlight the importance of the extraction conditions used that, without producing toxic molecules, allow us to obtain protecting molecules belonging to proanthocyanidin derivatives like procyanidin B2. In conclusion, we can hypothesize that HPTOPE, due to its functional and nontoxic properties on neuronal primary culture, can be utilized for future therapeutic interventions for neurodegeneration.
AB - We have recently demonstrated that bioactive molecules, extracted by high pressure and temperature from olive pomace, counteract calcium-induced cell damage to different cell lines. Here, our aim was to study the effect of the same extract on murine cortical neurons, since the preservation of the intracellular Ca2+-homeostasis is essential for neuronal function and survival. Accordingly, we treated neurons with different stimuli in order to evoke cytotoxic glutamatergic activation. In these conditions, the high-pressure and temperature extract from olive pomace (HPTOPE) only abolished the effects of N-methyl-d-aspartate (NMDA). Particularly, we observed that HPTOPE was able to promote the neuron rescue from NMDA-induced cell death. Moreover, we demonstrated that HPTOPE is endowed with the ability to maintain the intracellular Ca2+-homeostasis following NMDA receptor overactivation, protecting neurons from Ca2+-induced adverse effects, including aberrant calpain proteolytic activity. Moreover, we highlight the importance of the extraction conditions used that, without producing toxic molecules, allow us to obtain protecting molecules belonging to proanthocyanidin derivatives like procyanidin B2. In conclusion, we can hypothesize that HPTOPE, due to its functional and nontoxic properties on neuronal primary culture, can be utilized for future therapeutic interventions for neurodegeneration.
KW - Calcium homeostasis
KW - Calpain
KW - Neuron primary culture
KW - NMDAR
KW - Olive pomace extract
KW - Proanthocyanidins
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U2 - 10.3390/molecules25194385
DO - 10.3390/molecules25194385
M3 - Article
C2 - 32987671
AN - SCOPUS:85092023188
VL - 25
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 19
M1 - 4385
ER -