A bioassay of thyrotrophin by photografted mammalian cells onto polymeric supports

A. D'Ambrosio, I. R. Bellobono, F. Marangoni, P. Rebulla, G. Sirchia

Research output: Contribution to journalArticle

Abstract

Viable and functionally responsive human thyroid follicular cells, suspended in a commercial polyester acrylate diluted with tripropyleneglycol diacrylate, photoinitiated, and photocatalyzed with a proprietary photocatalytic system based on a synergic mixture of vanadium (V) t-butoxide and i-propoxide, have been immobilized as monolayers onto polystyrene plates. Bioassay of thyrotrophin in immobilized cell cultures yielded, by log-log plot of the dose-response curve, a slope (0.92 ± 0.02) in close agreement with that (0.91) reported for cells immobilized by physical adsorption. The decisive role of photocatalyst in the photografting procedure has also been shown experimentally. A mechanism is suggested by which cells are anchored, rapidly and with stable chemical bonds, onto one of the two acrylate functions of the monomer- prepolymer mixture, the other one being simultaneously responsible for photochemical grafting onto the support.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalBiotechnology and Bioengineering
Volume42
Issue number2
DOIs
Publication statusPublished - 1993

Keywords

  • immobilization
  • photocatalyst
  • photografting
  • thyroid cells
  • thyrotrophin bioassay

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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