A bone substitute composed of polymethylmethacrylate and α-tricalcium phosphate: Results in terms of osteoblast function and bone tissue formation

Milena Fini, Gianluca Giavaresi, Nicolo Nicoli Aldini, Paola Torricelli, Rodolfo Botter, Dario Beruto, Roberto Giardino

Research output: Contribution to journalArticle

Abstract

The biological properties of a composite polymeric matrix (PMMA+α-TCP) made of polymethylmethacrylate (PMMA) and alfa-tricalciumphosphate (α-TCP) was tested by means of in vitro and in vivo investigations. PMMA was used as a comparative material. Osteoblast cultures (MG 63) demonstrated that PMMA+α-TCP significantly and positively affected osteoblast viability, synthetic activity and interleukin-6 level as compared to PMMA. At 12 weeks, the PMMA+α-TCP implants in rabbit bone successfully osteointegrated in trabecular and cortical tissue (affinity index: 57.14±8.84% and 68.31±6.18%, respectively). The newly formed bone after tetracycline labelling was histologically observed inside PMMA+α-TCP porosity. The microhardness test at the bone-PMMA+α-TCP interface showed a significantly higher rate of newly formed bone mineralization compared with PMMA (+83.5% and +58.5%, respectively), but differences still existed between newly formed and pre-existing normal bone. It is herein hypothesized that the present positive results may be ascribed to the porous macroarchitecture of PMMA+α-TCP and the presence of the bioactive ceramic material that could have a synergic effect and be responsible for the improvement of (a) the material colonization by bone cells, (b) osteoblast activity, (c) osteoinduction and osteoconduction processes, (d) bone remodelling.

Original languageEnglish
Pages (from-to)4523-4531
Number of pages9
JournalBiomaterials
Volume23
Issue number23
DOIs
Publication statusPublished - Dec 2002

Keywords

  • α-tricalciumphosphate
  • Biocompatibility
  • Bone substitutes
  • Osteoblasts
  • Osteoinduction
  • Osteointegration
  • Polymethylmethacrylate

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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