A brief very-low oxygen tension regimen is sufficient for the early chondrogenic commitment of human adipose-derived mesenchymal stem cells

Marco Govoni, Claudio Muscari, Francesca Bonafè, Paolo Giovanni Morselli, Marilisa Cortesi, Dante Dallari, Emanuele Giordano

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE: The aim of this study was to evaluate the effects exerted over chondrogenic commitment of human adipose-derived mesenchymal stem cells (ADSCs) by a very low oxygen tension (<1% pO2).

MATERIALS/METHODS: Cell morphology, mRNA levels of chondrocyte-specific marker genes and the involvement of p38 MAPK signalling were monitored in human ADSCs under a very low oxygen tension.

RESULTS: Cell morphology was significantly changed after two days of hypoxic preconditioning when they featured as elongated spindle-shaped cells. SRY-box containing gene 9, aggrecan and collagen type II mRNA levels were enhanced under severe hypoxic culture conditions. Moreover, the inhibition of p38 MAPK resulted in a substantial reduction in transcription of the above-mentioned specific genes, proving the pivotal role of this pathway in the transcriptional regulation of chondrogenesis.

CONCLUSIONS: Here, we propose a protocol showing the early commitment of stem cells towards the chondrogenic phenotype in only 2 days of culture via a very low hypoxic environment, in the absence of growth factors added in the culture medium.

Original languageEnglish
Pages (from-to)98-104
Number of pages7
JournalAdvances in Medical Sciences
Volume66
Issue number1
DOIs
Publication statusE-pub ahead of print - Jan 15 2021

Keywords

  • Adipose-derived mesenchymal stem cells
  • Chondrogenic commitment
  • Hypoxia
  • Translational medicine
  • p38 MAPK

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