A Bronze-Tomato Enriched Diet Affects the Intestinal Microbiome under Homeostatic and Inflammatory Conditions

Marina Liso, Stefania De Santis, Aurelia Scarano, Giulio Verna, Manuela Dicarlo, Vanessa Galleggiante, Pietro Campiglia, Mauro Mastronardi, Antonio Lippolis, Mirco Vacca, Anastasia Sobolewski, Grazia Serino, Eugenio Butelli, Maria De Angelis, Cathie Martin, Angelo Santino, Marcello Chieppa

Research output: Contribution to journalArticle

Abstract

Inflammatory bowel diseases (IBD) are debilitating chronic inflammatory disorders that develop as a result of a defective immune response toward intestinal bacteria. Intestinal dysbiosis is associated with the onset of IBD and has been reported to persist even in patients in deep remission. We investigated the possibility of a dietary-induced switch to the gut microbiota composition using Winnie mice as a model of spontaneous ulcerative colitis and chow enriched with 1% Bronze tomato. We used the near isogenic tomato line strategy to investigate the effects of a diet enriched in polyphenols administered to mild but established chronic intestinal inflammation. The Bronze-enriched chow administered for two weeks was not able to produce any macroscopic effect on the IBD symptoms, although, at molecular level there was a significant induction of anti-inflammatory genes and intracellular staining of T cells revealed a mild decrease in IL17A and IFNγ production. Analysis of the microbial composition revealed that two weeks of Bronze enriched diet was sufficient to perturb the microbial composition of Winnie and control mice, suggesting that polyphenol-enriched diets may create unfavorable conditions for distinct bacterial species. In conclusion, dietary regimes enriched in polyphenols may efficiently support IBD remission affecting the intestinal dysbiosis.

Original languageEnglish
Pages (from-to)1862
Number of pages13
JournalNutrients
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 2 2018

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inflammatory bowel disease
Lycopersicon esculentum
Inflammatory Bowel Diseases
Polyphenols
Dysbiosis
tomatoes
Diet
polyphenols
remission
intestinal microorganisms
diet
colitis
mice
Ulcerative Colitis
signs and symptoms (animals and humans)
Anti-Inflammatory Agents
T-lymphocytes
inflammation
immune response
Staining and Labeling

Keywords

  • Animals
  • Anti-Inflammatory Agents/administration & dosage
  • Bacteria/classification
  • CD4-Positive T-Lymphocytes/metabolism
  • Colitis, Ulcerative/diet therapy
  • Diet
  • Disease Models, Animal
  • Dysbiosis/diet therapy
  • Fruit/chemistry
  • Gastrointestinal Microbiome/genetics
  • Inflammation/genetics
  • Inflammatory Bowel Diseases/diet therapy
  • Interferon-gamma/biosynthesis
  • Interleukin-17/biosynthesis
  • Lycopersicon esculentum/chemistry
  • Mice
  • Mice, Inbred C57BL
  • Polyphenols/administration & dosage
  • RNA, Ribosomal, 16S/analysis

Cite this

Liso, M., De Santis, S., Scarano, A., Verna, G., Dicarlo, M., Galleggiante, V., ... Chieppa, M. (2018). A Bronze-Tomato Enriched Diet Affects the Intestinal Microbiome under Homeostatic and Inflammatory Conditions. Nutrients, 10(12), 1862. https://doi.org/10.3390/nu10121862

A Bronze-Tomato Enriched Diet Affects the Intestinal Microbiome under Homeostatic and Inflammatory Conditions. / Liso, Marina; De Santis, Stefania; Scarano, Aurelia; Verna, Giulio; Dicarlo, Manuela; Galleggiante, Vanessa; Campiglia, Pietro; Mastronardi, Mauro; Lippolis, Antonio; Vacca, Mirco; Sobolewski, Anastasia; Serino, Grazia; Butelli, Eugenio; De Angelis, Maria; Martin, Cathie; Santino, Angelo; Chieppa, Marcello.

In: Nutrients, Vol. 10, No. 12, 02.12.2018, p. 1862.

Research output: Contribution to journalArticle

Liso, M, De Santis, S, Scarano, A, Verna, G, Dicarlo, M, Galleggiante, V, Campiglia, P, Mastronardi, M, Lippolis, A, Vacca, M, Sobolewski, A, Serino, G, Butelli, E, De Angelis, M, Martin, C, Santino, A & Chieppa, M 2018, 'A Bronze-Tomato Enriched Diet Affects the Intestinal Microbiome under Homeostatic and Inflammatory Conditions', Nutrients, vol. 10, no. 12, pp. 1862. https://doi.org/10.3390/nu10121862
Liso M, De Santis S, Scarano A, Verna G, Dicarlo M, Galleggiante V et al. A Bronze-Tomato Enriched Diet Affects the Intestinal Microbiome under Homeostatic and Inflammatory Conditions. Nutrients. 2018 Dec 2;10(12):1862. https://doi.org/10.3390/nu10121862
Liso, Marina ; De Santis, Stefania ; Scarano, Aurelia ; Verna, Giulio ; Dicarlo, Manuela ; Galleggiante, Vanessa ; Campiglia, Pietro ; Mastronardi, Mauro ; Lippolis, Antonio ; Vacca, Mirco ; Sobolewski, Anastasia ; Serino, Grazia ; Butelli, Eugenio ; De Angelis, Maria ; Martin, Cathie ; Santino, Angelo ; Chieppa, Marcello. / A Bronze-Tomato Enriched Diet Affects the Intestinal Microbiome under Homeostatic and Inflammatory Conditions. In: Nutrients. 2018 ; Vol. 10, No. 12. pp. 1862.
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abstract = "Inflammatory bowel diseases (IBD) are debilitating chronic inflammatory disorders that develop as a result of a defective immune response toward intestinal bacteria. Intestinal dysbiosis is associated with the onset of IBD and has been reported to persist even in patients in deep remission. We investigated the possibility of a dietary-induced switch to the gut microbiota composition using Winnie mice as a model of spontaneous ulcerative colitis and chow enriched with 1{\%} Bronze tomato. We used the near isogenic tomato line strategy to investigate the effects of a diet enriched in polyphenols administered to mild but established chronic intestinal inflammation. The Bronze-enriched chow administered for two weeks was not able to produce any macroscopic effect on the IBD symptoms, although, at molecular level there was a significant induction of anti-inflammatory genes and intracellular staining of T cells revealed a mild decrease in IL17A and IFNγ production. Analysis of the microbial composition revealed that two weeks of Bronze enriched diet was sufficient to perturb the microbial composition of Winnie and control mice, suggesting that polyphenol-enriched diets may create unfavorable conditions for distinct bacterial species. In conclusion, dietary regimes enriched in polyphenols may efficiently support IBD remission affecting the intestinal dysbiosis.",
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AU - Dicarlo, Manuela

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