A C1q solid phase microenzymatic assay for the detection of soluble immune complexes

Paola Cordiali Fei, Pier Giorgio Natali

Research output: Contribution to journalArticle

Abstract

The solid phase C1q-binding assay has been adapted to an enzymatic micromethod in which alkaline phosphatase labeled soluble Staphylococcus aureus protein A is used in place of the second antibody. The assay, which is run in microtiter plates, provides a rapid, sensitive (0.030 mg/ml of human heat-aggregated IgG detected) and reproducible method for the measurement of soluble immune complexes in a large number of samples. Soluble immune complexes prepared in vitro with bovine serum albumin (BSA) and anti-BSA antibodies on a wide range of antigen to antibody ratios were all detected with this method. When applied to the screening of unselected patient sera, soluble immune complexes were frequently found in systemic lupus erythematosus (52%) and chronic active hepatitis (57%) and in lower percentages in patients with malignant melanoma (28%), rheumatoid arthritis (30%) and essential mixed cryoglobulinemia (17%).

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalLa Ricerca in Clinica e in Laboratorio
Volume11
Issue number3
DOIs
Publication statusPublished - Jul 1981

Keywords

  • Autoimmune disorders
  • C1q solid phase
  • Enzymatic micromethod
  • Immune complexes
  • Malignant melanoma
  • Staphylococcal protein A

ASJC Scopus subject areas

  • Clinical Biochemistry

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