A carbon monoxide-releasing molecule (CORM-3) uncouples mitochondrial respiration and modulates the production of reactive oxygen species

Luisa Lo Iacono, Jorge Boczkowski, Roland Zini, Issam Salouage, Alain Berdeaux, Roberto Motterlini, Didier Morin

Research output: Contribution to journalArticle

Abstract

Carbon monoxide (CO), produced during the degradation of heme by the enzyme heme oxygenase, is an important signaling mediator in mammalian cells. Here we show that precise delivery of CO to isolated heart mitochondria using a water-soluble CO-releasing molecule (CORM-3) uncouples respiration. Addition of low-micromolar concentrations of CORM-3 (1-20 μM), but not an inactive compound that does not release CO, significantly increased mitochondrial oxygen consumption rate (State 2 respiration) in a concentration-dependent manner. In contrast, higher concentrations of CORM-3 (100 μM) suppressed ADP-dependent respiration through inhibition of cytochrome c oxidase. The uncoupling effect mediated by CORM-3 was inhibited in the presence of the CO scavenger myoglobin. Moreover, this effect was associated with a gradual decrease in membrane potential (ψ) over time and was partially reversed by malonate, an inhibitor of complex II activity. Similarly, inhibition of uncoupling proteins or blockade of adenine nucleotide transporter attenuated the effect of CORM-3 on both State 2 respiration and Δψ. Hydrogen peroxide (H 2O2) produced by mitochondria respiring from complex I-linked substrates (pyruvate/malate) was increased by CORM-3. However, respiration initiated via complex II using succinate resulted in a fivefold increase in H2O2 production and this effect was significantly inhibited by CORM-3. These findings disclose a counterintuitive action of CORM-3 suggesting that CO at low levels acts as an important regulator of mitochondrial respiration.

Original languageEnglish
Pages (from-to)1556-1564
Number of pages9
JournalFree Radical Biology and Medicine
Volume50
Issue number11
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Carbon Monoxide
Reactive Oxygen Species
Respiration
Molecules
Mitochondria
Heme Oxygenase (Decyclizing)
Heart Mitochondria
Adenine Nucleotides
Myoglobin
Succinic Acid
Electron Transport Complex IV
Pyruvic Acid
Heme
Oxygen Consumption
Membrane Potentials
Adenosine Diphosphate
Hydrogen Peroxide
Cells
Oxygen
Membranes

Keywords

  • Carbon monoxide
  • CO-releasing molecules
  • Free radicals
  • Mitochondrial respiration
  • Reactive oxygen species
  • Uncoupling agents

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

A carbon monoxide-releasing molecule (CORM-3) uncouples mitochondrial respiration and modulates the production of reactive oxygen species. / Lo Iacono, Luisa; Boczkowski, Jorge; Zini, Roland; Salouage, Issam; Berdeaux, Alain; Motterlini, Roberto; Morin, Didier.

In: Free Radical Biology and Medicine, Vol. 50, No. 11, 01.06.2011, p. 1556-1564.

Research output: Contribution to journalArticle

Lo Iacono, Luisa ; Boczkowski, Jorge ; Zini, Roland ; Salouage, Issam ; Berdeaux, Alain ; Motterlini, Roberto ; Morin, Didier. / A carbon monoxide-releasing molecule (CORM-3) uncouples mitochondrial respiration and modulates the production of reactive oxygen species. In: Free Radical Biology and Medicine. 2011 ; Vol. 50, No. 11. pp. 1556-1564.
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