A CD3+CD8+ T Cell Population Lacking CD5 Antigen Expression Is Expanded in Peripheral Blood of Human Immunodeficiency Virus-Infected Patients

Stefano Indraccolo, Marta Mion, Rita Zamarchi, Vincenzo Coppola, Francesca Calderazzo, Alberto Amadori, Luigi Chieco-Bianchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this study we analyzed the behavior of a CD3+ T cell subpopulation lacking CD5 antigen expression in PBMC from HIV-1-infected patients. CD3+CD5- lymphocytes were greatly increased in peripheral blood of HIV-1+ patients, accounting for 20.6 ± 9.9% of the total CD3+ cells, compared to seronegative individuals (5.5 ± 3.2%). In both seropositive patients and controls, CD3+CD5- cells belonged to the CD8+ compartment; they were nonactivated, TCR α/β+, naive lymphocytes, and in seronegative individuals preferentially expressed NK cell-associated markers, such as CD11b, CD16, CD56, and CD57. The phenotypic profile of this subset was slightly different in seropositive patients; while TCR expression and CD45RA/RO profile were comparable, CD11b and CD16 expression was lower compared to control figures, while CD56 expression was not changed, and CD57 expression was enhanced. Functional analysis of enriched CD3+CD8+CD5- cells showed an impaired ability to proliferate in response to mitogenic and antigenic stimuli; despite their NKlike phenotype, CD3+CD8+CD5- cells did not exert any NK cytotoxic activity, and only a lectin-dependent cytotoxic potential could be evidenced in this population. These results describe a novel alteration in the lymphocyte phenotypic profile during HIV-1 infection, involving a "transitional" population, which shares some properties of the T and of the NK cell lineage.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalClinical Immunology and Immunopathology
Volume77
Issue number3
DOIs
Publication statusPublished - Dec 1995

Fingerprint

CD5 Antigens
HIV
T-Lymphocytes
HIV-1
Lymphocytes
Natural Killer Cells
Population
Cell Lineage
Lectins
HIV Infections
Phenotype

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Immunology

Cite this

A CD3+CD8+ T Cell Population Lacking CD5 Antigen Expression Is Expanded in Peripheral Blood of Human Immunodeficiency Virus-Infected Patients. / Indraccolo, Stefano; Mion, Marta; Zamarchi, Rita; Coppola, Vincenzo; Calderazzo, Francesca; Amadori, Alberto; Chieco-Bianchi, Luigi.

In: Clinical Immunology and Immunopathology, Vol. 77, No. 3, 12.1995, p. 253-261.

Research output: Contribution to journalArticle

@article{cadf2b9a94f34c6d80b883987b86804d,
title = "A CD3+CD8+ T Cell Population Lacking CD5 Antigen Expression Is Expanded in Peripheral Blood of Human Immunodeficiency Virus-Infected Patients",
abstract = "In this study we analyzed the behavior of a CD3+ T cell subpopulation lacking CD5 antigen expression in PBMC from HIV-1-infected patients. CD3+CD5- lymphocytes were greatly increased in peripheral blood of HIV-1+ patients, accounting for 20.6 ± 9.9{\%} of the total CD3+ cells, compared to seronegative individuals (5.5 ± 3.2{\%}). In both seropositive patients and controls, CD3+CD5- cells belonged to the CD8+ compartment; they were nonactivated, TCR α/β+, naive lymphocytes, and in seronegative individuals preferentially expressed NK cell-associated markers, such as CD11b, CD16, CD56, and CD57. The phenotypic profile of this subset was slightly different in seropositive patients; while TCR expression and CD45RA/RO profile were comparable, CD11b and CD16 expression was lower compared to control figures, while CD56 expression was not changed, and CD57 expression was enhanced. Functional analysis of enriched CD3+CD8+CD5- cells showed an impaired ability to proliferate in response to mitogenic and antigenic stimuli; despite their NKlike phenotype, CD3+CD8+CD5- cells did not exert any NK cytotoxic activity, and only a lectin-dependent cytotoxic potential could be evidenced in this population. These results describe a novel alteration in the lymphocyte phenotypic profile during HIV-1 infection, involving a {"}transitional{"} population, which shares some properties of the T and of the NK cell lineage.",
author = "Stefano Indraccolo and Marta Mion and Rita Zamarchi and Vincenzo Coppola and Francesca Calderazzo and Alberto Amadori and Luigi Chieco-Bianchi",
year = "1995",
month = "12",
doi = "10.1006/clin.1995.1151",
language = "English",
volume = "77",
pages = "253--261",
journal = "Clinical Immunology and Immunopathology",
issn = "0090-1229",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A CD3+CD8+ T Cell Population Lacking CD5 Antigen Expression Is Expanded in Peripheral Blood of Human Immunodeficiency Virus-Infected Patients

AU - Indraccolo, Stefano

AU - Mion, Marta

AU - Zamarchi, Rita

AU - Coppola, Vincenzo

AU - Calderazzo, Francesca

AU - Amadori, Alberto

AU - Chieco-Bianchi, Luigi

PY - 1995/12

Y1 - 1995/12

N2 - In this study we analyzed the behavior of a CD3+ T cell subpopulation lacking CD5 antigen expression in PBMC from HIV-1-infected patients. CD3+CD5- lymphocytes were greatly increased in peripheral blood of HIV-1+ patients, accounting for 20.6 ± 9.9% of the total CD3+ cells, compared to seronegative individuals (5.5 ± 3.2%). In both seropositive patients and controls, CD3+CD5- cells belonged to the CD8+ compartment; they were nonactivated, TCR α/β+, naive lymphocytes, and in seronegative individuals preferentially expressed NK cell-associated markers, such as CD11b, CD16, CD56, and CD57. The phenotypic profile of this subset was slightly different in seropositive patients; while TCR expression and CD45RA/RO profile were comparable, CD11b and CD16 expression was lower compared to control figures, while CD56 expression was not changed, and CD57 expression was enhanced. Functional analysis of enriched CD3+CD8+CD5- cells showed an impaired ability to proliferate in response to mitogenic and antigenic stimuli; despite their NKlike phenotype, CD3+CD8+CD5- cells did not exert any NK cytotoxic activity, and only a lectin-dependent cytotoxic potential could be evidenced in this population. These results describe a novel alteration in the lymphocyte phenotypic profile during HIV-1 infection, involving a "transitional" population, which shares some properties of the T and of the NK cell lineage.

AB - In this study we analyzed the behavior of a CD3+ T cell subpopulation lacking CD5 antigen expression in PBMC from HIV-1-infected patients. CD3+CD5- lymphocytes were greatly increased in peripheral blood of HIV-1+ patients, accounting for 20.6 ± 9.9% of the total CD3+ cells, compared to seronegative individuals (5.5 ± 3.2%). In both seropositive patients and controls, CD3+CD5- cells belonged to the CD8+ compartment; they were nonactivated, TCR α/β+, naive lymphocytes, and in seronegative individuals preferentially expressed NK cell-associated markers, such as CD11b, CD16, CD56, and CD57. The phenotypic profile of this subset was slightly different in seropositive patients; while TCR expression and CD45RA/RO profile were comparable, CD11b and CD16 expression was lower compared to control figures, while CD56 expression was not changed, and CD57 expression was enhanced. Functional analysis of enriched CD3+CD8+CD5- cells showed an impaired ability to proliferate in response to mitogenic and antigenic stimuli; despite their NKlike phenotype, CD3+CD8+CD5- cells did not exert any NK cytotoxic activity, and only a lectin-dependent cytotoxic potential could be evidenced in this population. These results describe a novel alteration in the lymphocyte phenotypic profile during HIV-1 infection, involving a "transitional" population, which shares some properties of the T and of the NK cell lineage.

UR - http://www.scopus.com/inward/record.url?scp=0028784022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028784022&partnerID=8YFLogxK

U2 - 10.1006/clin.1995.1151

DO - 10.1006/clin.1995.1151

M3 - Article

C2 - 7586735

AN - SCOPUS:0028784022

VL - 77

SP - 253

EP - 261

JO - Clinical Immunology and Immunopathology

JF - Clinical Immunology and Immunopathology

SN - 0090-1229

IS - 3

ER -