A CDC6-like factor from the archaea Sulfolobus solfataricus promotes binding of the mini-chromosome maintenance complex to DNA

Mariarita De Felice, Luca Esposito, Biagio Pucci, Mariarosaria De Falco, Mosè Rossi, Francesca M. Pisani

Research output: Contribution to journalArticlepeer-review

Abstract

The archaeal replication apparatus appears to be a simplified version of the eukaryotic one with fewer polypeptides and simpler protein complexes. Herein, we report evidence that a Cdc6-like factor from the hyperthermophilic crenarchaea Sulfolobus solfataricus stimulates binding of the homohexameric MCM-like complex to bubble- and fork-containing DNA oligonucleotides that mimic early replication intermediates. This function does not require the Cdc6 ATP and DNA binding activities. These findings may provide important clues to understanding how the DNA replication initiation process has evolved in the more complex eukaryotic organisms.

Original languageEnglish
Pages (from-to)43008-43012
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number41
DOIs
Publication statusPublished - Oct 8 2004

ASJC Scopus subject areas

  • Biochemistry

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