A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts: Arrest and dissociation between uptake of thymidine and DNA synthesis

L. Mallucci, M. Dunn, V. Wells, D. Delia

Research output: Contribution to journalArticle

Abstract

We have examined the effects of 50 μg ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of growth not solely by preventing G1-G0 cells from entering the S-phase but also by exerting a G2 block. We also found that Con A, which prevented commencement of S-phase, did not arrest cells already in S from reaching the G2 stage but inhibited the S-phase associated process of thymidine uptake. The inhibition was greater when the Con A receptors were extensively clustered.

Original languageEnglish
Pages (from-to)353-363
Number of pages11
JournalJournal of Cell Science
VolumeVOL.46
Publication statusPublished - 1980

Fingerprint

S Phase
Thymidine
Cell Cycle
Embryonic Structures
Fibroblasts
DNA
Growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts : Arrest and dissociation between uptake of thymidine and DNA synthesis. / Mallucci, L.; Dunn, M.; Wells, V.; Delia, D.

In: Journal of Cell Science, Vol. VOL.46, 1980, p. 353-363.

Research output: Contribution to journalArticle

@article{0ec6670934e843f8a93ed4703f4098c2,
title = "A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts: Arrest and dissociation between uptake of thymidine and DNA synthesis",
abstract = "We have examined the effects of 50 μg ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of growth not solely by preventing G1-G0 cells from entering the S-phase but also by exerting a G2 block. We also found that Con A, which prevented commencement of S-phase, did not arrest cells already in S from reaching the G2 stage but inhibited the S-phase associated process of thymidine uptake. The inhibition was greater when the Con A receptors were extensively clustered.",
author = "L. Mallucci and M. Dunn and V. Wells and D. Delia",
year = "1980",
language = "English",
volume = "VOL.46",
pages = "353--363",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",

}

TY - JOUR

T1 - A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts

T2 - Arrest and dissociation between uptake of thymidine and DNA synthesis

AU - Mallucci, L.

AU - Dunn, M.

AU - Wells, V.

AU - Delia, D.

PY - 1980

Y1 - 1980

N2 - We have examined the effects of 50 μg ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of growth not solely by preventing G1-G0 cells from entering the S-phase but also by exerting a G2 block. We also found that Con A, which prevented commencement of S-phase, did not arrest cells already in S from reaching the G2 stage but inhibited the S-phase associated process of thymidine uptake. The inhibition was greater when the Con A receptors were extensively clustered.

AB - We have examined the effects of 50 μg ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of growth not solely by preventing G1-G0 cells from entering the S-phase but also by exerting a G2 block. We also found that Con A, which prevented commencement of S-phase, did not arrest cells already in S from reaching the G2 stage but inhibited the S-phase associated process of thymidine uptake. The inhibition was greater when the Con A receptors were extensively clustered.

UR - http://www.scopus.com/inward/record.url?scp=0019192003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019192003&partnerID=8YFLogxK

M3 - Article

C2 - 7228912

AN - SCOPUS:0019192003

VL - VOL.46

SP - 353

EP - 363

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -