A cellular system to study the role of nitric oxide in cell death, survival, and migration

Stefania Bulotta, Cristiana Perrotta, Andrea Cerullo, Clara De Palma, Emilio Clementi, Nica Borgese

Research output: Contribution to journalArticlepeer-review

Abstract

The gaseous messenger nitric oxide (NO) plays a bewildering number of roles in fundamental processes, such as cell locomotion, differentiation, proliferation and death. Its different and often contrasting roles may depend on its concentration and intracellular site of generation. We describe here a simple system with which to investigate the roles of NO generated at physiological levels in HeLa cells by eNOS transfected under an inducible promoter. This system has allowed us to uncover unexpected signalling circuits between NO and ceramide, involved in the response of cells to apoptotic stimuli. At present, we are using these cells as a tool to investigate the role of NO in migration.

Original languageEnglish
Pages (from-to)841-845
Number of pages5
JournalNeuroToxicology
Volume26
Issue number5
DOIs
Publication statusPublished - Oct 2005

Keywords

  • Apoptosis
  • Ceramide
  • Endothelial NO synthase
  • PI3 kinase/Akt
  • TetOff system

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Toxicology

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