Abstract
Because of the fact that tumor cell sensitivity to cytotoxic agents may play a major role in cancer treatment, and several anthracyclines are widely used for first-line treatment of leukemia, lymphoma and other tumors, and since the overexpression of the mdr-1 gene-coded 170 Kd glycoprotein (P170) decreases cell sensitivity to anthracyclines, we investigated the relationship between P170 overexpression and the cytotoxicity of two classic anthracyclines (Daunorubicin or DNR and Doxorubicin or DX) and two lipophilic anthracycline derivatives (Idarubicin or IDA and Iododoxorubicin or IDX). For these purposes, we used multidrug resistant (MDR) and non-MDR tumor and leukemia cell lines and the MTT-microcultured tetrazolium colorimetric assay. We showed that mdr-1 gene overexpression was strongly associated with the development of a high level of resistance to DNR and DX, but not to the derivatives IDA and IDX. These data suggest that more lipophilic anthracycline derivatives may also be active in MDR cell systems.
| Original language | English |
|---|---|
| Pages (from-to) | 255-264 |
| Number of pages | 10 |
| Journal | Leukemia and Lymphoma |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1993 |
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Keywords
- Anthracyclines
- Chemotherapy
- Multidrug resistance
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research
Cite this
A comparative analysis of the sensitivity of multidrug resistant (MDR) and non-MDR cells to different anthracycline derivatives. / Michieli, Mariagrazia; Michelutti, Angela; Damiani, Daniela; Pipan, Corrado; Raspadori, Donatella; Lauria, Francesco; Baccarani, Michele.
In: Leukemia and Lymphoma, Vol. 9, No. 3, 1993, p. 255-264.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A comparative analysis of the sensitivity of multidrug resistant (MDR) and non-MDR cells to different anthracycline derivatives
AU - Michieli, Mariagrazia
AU - Michelutti, Angela
AU - Damiani, Daniela
AU - Pipan, Corrado
AU - Raspadori, Donatella
AU - Lauria, Francesco
AU - Baccarani, Michele
PY - 1993
Y1 - 1993
N2 - Because of the fact that tumor cell sensitivity to cytotoxic agents may play a major role in cancer treatment, and several anthracyclines are widely used for first-line treatment of leukemia, lymphoma and other tumors, and since the overexpression of the mdr-1 gene-coded 170 Kd glycoprotein (P170) decreases cell sensitivity to anthracyclines, we investigated the relationship between P170 overexpression and the cytotoxicity of two classic anthracyclines (Daunorubicin or DNR and Doxorubicin or DX) and two lipophilic anthracycline derivatives (Idarubicin or IDA and Iododoxorubicin or IDX). For these purposes, we used multidrug resistant (MDR) and non-MDR tumor and leukemia cell lines and the MTT-microcultured tetrazolium colorimetric assay. We showed that mdr-1 gene overexpression was strongly associated with the development of a high level of resistance to DNR and DX, but not to the derivatives IDA and IDX. These data suggest that more lipophilic anthracycline derivatives may also be active in MDR cell systems.
AB - Because of the fact that tumor cell sensitivity to cytotoxic agents may play a major role in cancer treatment, and several anthracyclines are widely used for first-line treatment of leukemia, lymphoma and other tumors, and since the overexpression of the mdr-1 gene-coded 170 Kd glycoprotein (P170) decreases cell sensitivity to anthracyclines, we investigated the relationship between P170 overexpression and the cytotoxicity of two classic anthracyclines (Daunorubicin or DNR and Doxorubicin or DX) and two lipophilic anthracycline derivatives (Idarubicin or IDA and Iododoxorubicin or IDX). For these purposes, we used multidrug resistant (MDR) and non-MDR tumor and leukemia cell lines and the MTT-microcultured tetrazolium colorimetric assay. We showed that mdr-1 gene overexpression was strongly associated with the development of a high level of resistance to DNR and DX, but not to the derivatives IDA and IDX. These data suggest that more lipophilic anthracycline derivatives may also be active in MDR cell systems.
KW - Anthracyclines
KW - Chemotherapy
KW - Multidrug resistance
UR - http://www.scopus.com/inward/record.url?scp=0027322641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027322641&partnerID=8YFLogxK
U2 - 10.3109/10428199309147379
DO - 10.3109/10428199309147379
M3 - Article
VL - 9
SP - 255
EP - 264
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
SN - 1042-8194
IS - 3
ER -