A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins

M. Coletta, M. E. Clementi, P. Ascenzi, R. Petruzzelli, S. G. Condo, B. Giardina

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The effect of temperature on the oxygen-binding properties of hemoglobin (Hb) from ruminants, such as ox, reindeer, musk ox, mouflon and egyptian water buffalo is compared to that of human adult Hb (HbA). A striking difference emerges where in the presence of chloride ions and in the absence of 2,3-diphosphoglycerate [Gri(2,3)P2] a strongly reduced exothermic oxygenation process is observed for all ruminant Hb investigated with respect to HbA. Next, in the presence of physiological concentrations of Gri(2,3)P2, HbA displays a less exothermic oxygenation process, with values tending toward those observed in ruminant Hb [where Gri(2,3)P2 is not a physiological effector and for which the addition of Gri(2,3)P2 has essentially no effect on the oxygenation enthalpy]. Different from HbA, the intrinsically less exothermic oxygen binding seems to be independent of the experimental conditions for ruminant Hb, underlying specific structural characteristics which might be responsible for this feature.

Original languageEnglish
Pages (from-to)1155-1157
Number of pages3
JournalEuropean Journal of Biochemistry
Volume204
Issue number3
Publication statusPublished - 1992

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Ruminants
Hemoglobins
Oxygenation
Oxygen
Temperature
Reindeer
Domestic Sheep
2,3-Diphosphoglycerate
Buffaloes
Chlorides
Enthalpy
Ions
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Coletta, M., Clementi, M. E., Ascenzi, P., Petruzzelli, R., Condo, S. G., & Giardina, B. (1992). A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins. European Journal of Biochemistry, 204(3), 1155-1157.

A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins. / Coletta, M.; Clementi, M. E.; Ascenzi, P.; Petruzzelli, R.; Condo, S. G.; Giardina, B.

In: European Journal of Biochemistry, Vol. 204, No. 3, 1992, p. 1155-1157.

Research output: Contribution to journalArticle

Coletta, M, Clementi, ME, Ascenzi, P, Petruzzelli, R, Condo, SG & Giardina, B 1992, 'A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins', European Journal of Biochemistry, vol. 204, no. 3, pp. 1155-1157.
Coletta M, Clementi ME, Ascenzi P, Petruzzelli R, Condo SG, Giardina B. A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins. European Journal of Biochemistry. 1992;204(3):1155-1157.
Coletta, M. ; Clementi, M. E. ; Ascenzi, P. ; Petruzzelli, R. ; Condo, S. G. ; Giardina, B. / A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins. In: European Journal of Biochemistry. 1992 ; Vol. 204, No. 3. pp. 1155-1157.
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