TY - JOUR
T1 - A Comparative Transcriptional Map of a Region of 250 kb on the Human and Mouse X Chromosome between the G6PD and the FLN1 Genes
AU - Rivella, Stefano
AU - Tamanini, Filippo
AU - Bione, Silvia
AU - Mancini, Mita
AU - Herman, Gail
AU - Chatterjee, Aurobindo
AU - Maestrini, Elena
AU - Toniolo, Daniela
PY - 1995/8/10
Y1 - 1995/8/10
N2 - The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with whole cosmids. Overlapping cosmids were aligned by comparing EcoRI and rare-cutter restriction enzyme digestions. The gene order and the orientation of transcription were determined by hybridization with fragments from the 5′ and 3′ moieties of each cDNA. Our work demonstrates that all of the new genes identified in human are present in the mouse. The size of the region, 250 kb, is also very similar, as are gene order and gene organization: the transcriptional organization in "domains" described in human is found to be identical in the mouse. The major difference detected is the much lower content in rare-cutter restriction sites, which is related to the lower G+C and CpG content of mouse DNA. The very high conservation that we have described suggests that a potent selective pressure has contributed to such conservation of gene organization.
AB - The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with whole cosmids. Overlapping cosmids were aligned by comparing EcoRI and rare-cutter restriction enzyme digestions. The gene order and the orientation of transcription were determined by hybridization with fragments from the 5′ and 3′ moieties of each cDNA. Our work demonstrates that all of the new genes identified in human are present in the mouse. The size of the region, 250 kb, is also very similar, as are gene order and gene organization: the transcriptional organization in "domains" described in human is found to be identical in the mouse. The major difference detected is the much lower content in rare-cutter restriction sites, which is related to the lower G+C and CpG content of mouse DNA. The very high conservation that we have described suggests that a potent selective pressure has contributed to such conservation of gene organization.
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U2 - 10.1006/geno.1995.1164
DO - 10.1006/geno.1995.1164
M3 - Article
C2 - 7490070
AN - SCOPUS:0029100314
VL - 28
SP - 377
EP - 382
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -