TY - JOUR
T1 - A comparison between radiometric and fluorimetric methods for measuring SSAO activity
AU - Gella, Alejandro
AU - Solé, Montse
AU - Bolea, Irene
AU - Ventriglia, Mariacarla
AU - Siotto, Mariacristina
AU - Durany, Nuria
AU - Squitti, Rosanna
AU - Unzeta, Mercedes
PY - 2013/6
Y1 - 2013/6
N2 - Semicarbazide-sensitive amine oxidase (SSAO) metabolizes the oxidative deamination of primary aromatic and aliphatic amines. The final cytotoxic products of its catalysis contribute to diseases involving vascular degeneration. The increasing interest in measuring SSAO activity has led to the development of several different methods. Herein, we compare SSAO activity results obtained with radiometric and fluorimetric methods in 49 plasma samples. Although not interchangeable, a significant correlation was obtained between methods. Considering these limitations, the fluorimetric method might replace the radioisotopic one.
AB - Semicarbazide-sensitive amine oxidase (SSAO) metabolizes the oxidative deamination of primary aromatic and aliphatic amines. The final cytotoxic products of its catalysis contribute to diseases involving vascular degeneration. The increasing interest in measuring SSAO activity has led to the development of several different methods. Herein, we compare SSAO activity results obtained with radiometric and fluorimetric methods in 49 plasma samples. Although not interchangeable, a significant correlation was obtained between methods. Considering these limitations, the fluorimetric method might replace the radioisotopic one.
KW - Biomarkers
KW - Fluorimetric
KW - Method comparison
KW - Radiometric
KW - Semicarbazide- sensitive amine oxidase
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U2 - 10.1007/s00702-013-0987-z
DO - 10.1007/s00702-013-0987-z
M3 - Article
C2 - 23400361
AN - SCOPUS:84878731782
VL - 120
SP - 1015
EP - 1018
JO - Journal of Neuro-Visceral Relations
JF - Journal of Neuro-Visceral Relations
SN - 0375-9245
IS - 6
ER -