TY - JOUR
T1 - A complex prediction
T2 - Three-dimensional model of the yeast exosome
AU - Aloy, Patrick
AU - Ciccarelli, Francesca D.
AU - Leutwein, Christina
AU - Gavin, Anne Claude
AU - Superti-Furga, Giulio
AU - Bork, Peer
AU - Böttcher, Bettina
AU - Russell, Robert B.
PY - 2002
Y1 - 2002
N2 - We present a model of the yeast exosome based on the bacterial degradosome component polynucleotide phosphorylase (PNPase). Electron microscopy shows the exosome to resemble PNPase but with key differences likely related to the position of RNA binding domains, and to the location of domains unique to the exosome. We use various techniques to reduce the many possible models of exosome subunits based on PNPase to just one. The model suggests numerous experiments to probe exosome function, particularly with respect to subunits making direct atomic contacts and conserved, possibly functional residues within the predicted central pore of the complex.
AB - We present a model of the yeast exosome based on the bacterial degradosome component polynucleotide phosphorylase (PNPase). Electron microscopy shows the exosome to resemble PNPase but with key differences likely related to the position of RNA binding domains, and to the location of domains unique to the exosome. We use various techniques to reduce the many possible models of exosome subunits based on PNPase to just one. The model suggests numerous experiments to probe exosome function, particularly with respect to subunits making direct atomic contacts and conserved, possibly functional residues within the predicted central pore of the complex.
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U2 - 10.1093/embo-reports/kvf135
DO - 10.1093/embo-reports/kvf135
M3 - Article
C2 - 12101094
AN - SCOPUS:0036023943
VL - 3
SP - 628
EP - 635
JO - EMBO Reports
JF - EMBO Reports
SN - 1469-221X
IS - 7
ER -