A complex prediction: Three-dimensional model of the yeast exosome

Patrick Aloy, Francesca D. Ciccarelli, Christina Leutwein, Anne Claude Gavin, Giulio Superti-Furga, Peer Bork, Bettina Böttcher, Robert B. Russell

Research output: Contribution to journalArticlepeer-review


We present a model of the yeast exosome based on the bacterial degradosome component polynucleotide phosphorylase (PNPase). Electron microscopy shows the exosome to resemble PNPase but with key differences likely related to the position of RNA binding domains, and to the location of domains unique to the exosome. We use various techniques to reduce the many possible models of exosome subunits based on PNPase to just one. The model suggests numerous experiments to probe exosome function, particularly with respect to subunits making direct atomic contacts and conserved, possibly functional residues within the predicted central pore of the complex.

Original languageEnglish
Pages (from-to)628-635
Number of pages8
JournalEMBO Reports
Issue number7
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Cell Biology
  • Genetics


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