A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration

Alice Roffi, Elizaveta Kon, Francesco Perdisa, Milena Fini, Alessandro Di Martino, Annapaola Parrilli, Francesca Salamanna, Monica Sandri, Maria Sartori, Simone Sprio, Anna Tampieri, Maurilio Marcacci, Giuseppe Filardo

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Abstract

Several biomaterials have recently been developed to address the challenge of osteochondral regeneration. Among these, chitosan holds promises both for cartilage and bone healing. The aim of this in vivo study was to evaluate the regeneration potential of a novel hybrid magnesium-doped hydroxyapatite (MgHA), collagen, chitosan-based scaffold, which was tested in a sheep model to ascertain its osteochondral regenerative potential, and in a rabbit model to further evaluate its ability to regenerate bone tissue. Macroscopic, microtomography, histology, histomorphometry, and immunohistochemical analysis were performed. In the sheep model, all analyses did not show significant differences compared to untreated defects (p > 0.05), with no evidence of cartilage and subchondral bone regeneration. In the rabbit model, this bone scaffold provided less ability to enhance tissue healing compared with a commercial bone scaffold. Moreover, persistence of scaffold material and absence of integration with connective tissue around the scaffolds were observed. These results raised some concerns about the osteochondral use of this chitosan composite scaffold, especially for the bone layer. Further studies are needed to explore the best formulation of chitosan-reinforced composites for osteochondral treatment.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume20
Issue number9
DOIs
Publication statusPublished - May 7 2019

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Keywords

  • bone
  • cartilage
  • chitosan
  • failure
  • osteochondral
  • scaffold

Cite this

Roffi, A., Kon, E., Perdisa, F., Fini, M., Di Martino, A., Parrilli, A., Salamanna, F., Sandri, M., Sartori, M., Sprio, S., Tampieri, A., Marcacci, M., & Filardo, G. (2019). A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration. International Journal of Molecular Sciences, 20(9), 1-14. https://doi.org/10.3390/ijms20092227