A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen helicobacter pylori

Andrea Vannini, Francesca Agriesti, Flaviana Mosca, Davide Roncarati, Vincenzo Scarlato, Alberto Danielli

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studies. Here, we report the construction of a bioluminescent H. pylori reporter system based on the Photorhabdus luminescens luxCDABE cassette. The system is constituted of a promoterless lux acceptor strain in which promoters and sequences of interest can be conveniently introduced by double homologous recombination of a suicide transformation vector. We validate the robustness of this new lux reporter system in noninvasive in vivo monitoring of dynamic transcriptional responses of inducible as well as repressible promoters and demonstrate its suitability for the implementation of genetic screens in H. pylori.

Original languageEnglish
Pages (from-to)6524-6533
Number of pages10
JournalApplied and Environmental Microbiology
Volume78
Issue number18
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Helicobacter pylori
gene expression
pathogen
Gene Expression
suicide
pathogens
monitoring
Photorhabdus
dynamic response
Photorhabdus luminescens
promoter regions
recombination
Homologous Recombination
homologous recombination
Suicide
pathogenesis
Biological Sciences
Research

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen helicobacter pylori. / Vannini, Andrea; Agriesti, Francesca; Mosca, Flaviana; Roncarati, Davide; Scarlato, Vincenzo; Danielli, Alberto.

In: Applied and Environmental Microbiology, Vol. 78, No. 18, 09.2012, p. 6524-6533.

Research output: Contribution to journalArticle

Vannini, Andrea ; Agriesti, Francesca ; Mosca, Flaviana ; Roncarati, Davide ; Scarlato, Vincenzo ; Danielli, Alberto. / A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen helicobacter pylori. In: Applied and Environmental Microbiology. 2012 ; Vol. 78, No. 18. pp. 6524-6533.
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