@inbook{78a184de2355445fb76607121181425c,
title = "A cytofluorimetric assay to evaluate T cell polyfunctionality",
abstract = "The magnitude and the quality of T-cell response are critical endpoints in the immune-monitoring of cancer patients. The release of cytokines and lytic factors by T cells operate in each phase of the cancer immunity cycle. The simultaneous cytokine production, defined as T-cell polyfunctionality, is a dynamic state of different T-cell subsets and may represent a surrogate biomarker of response to immune-mediated therapies. To quantify the T-cell immune mediators, different methodologies are available. Herein we describe two flow cytometry-based protocols to detect the simultaneous intracellular cytokine production, by using different methods of T-cell activation. Among the different procedures, multicolor flow cytometry is considered a powerful, quick, and semiquantitative technique, able to achieve the analysis of both surface and intracellular proteins, expressed by specific T-cell subsets. We report the capability of this technique to study simultaneous cytokine production within a heterogeneous population. As the field of novel single-cell high-throughput methodological approaches advance in this exciting era of Immuno-Oncology, we expect to gather even more information on the immunodynamics of polyfunctional T cells and their role in improving disease control.",
keywords = "Cytokines, Flow cytometry, Inflammatory mediators, Lytic granules, Monensin, Polyfunctionality, T cells, intracellular staining",
author = "Belinda Palermo and Mariangela Panetta and Giulia Campo and Paola Nistic{\`o}",
year = "2019",
month = jan,
day = "1",
doi = "10.1016/bs.mie.2019.07.041",
language = "English",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
booktitle = "Methods in Enzymology",
address = "United States",
}