A cytokeratin-immunohistochemical study of hepatoblastoma

Peter Van Eyken; Raf Sciot; Francesco Callea; Frans Ramaekers; G. Schaart; Valeer J. Desmet

doi:10.1016/0046-8177(90)90231-S

A cytokeratin-immunohistochemical study of hepatoblastoma

Peter Van Eyken, Raf Sciot, Francesco Callea, Frans Ramaekers, G. Schaart, Valeer J. Desmet

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article

Abstract

Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.

Original language	English
Pages (from-to)	302-308
Number of pages	7
Journal	Human Pathology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/0046-8177(90)90231-S
Publication status	Published - 1990

Keywords

cytokeratins
hepatoblastoma
histogenesis
immunohistochemistry

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1016/0046-8177(90)90231-S

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Van Eyken, P., Sciot, R., Callea, F., Ramaekers, F., Schaart, G., & Desmet, V. J. (1990). A cytokeratin-immunohistochemical study of hepatoblastoma. Human Pathology, 21(3), 302-308. https://doi.org/10.1016/0046-8177(90)90231-S

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AU - Ramaekers, Frans

AU - Schaart, G.

AU - Desmet, Valeer J.

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N2 - Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.

AB - Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.

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