Abstract
Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.
| Original language | English |
|---|---|
| Pages (from-to) | 302-308 |
| Number of pages | 7 |
| Journal | Human Pathology |
| Volume | 21 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1990 |
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Keywords
- cytokeratins
- hepatoblastoma
- histogenesis
- immunohistochemistry
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
A cytokeratin-immunohistochemical study of hepatoblastoma. / Van Eyken, Peter; Sciot, Raf; Callea, Francesco; Ramaekers, Frans; Schaart, G.; Desmet, Valeer J.
In: Human Pathology, Vol. 21, No. 3, 1990, p. 302-308.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A cytokeratin-immunohistochemical study of hepatoblastoma
AU - Van Eyken, Peter
AU - Sciot, Raf
AU - Callea, Francesco
AU - Ramaekers, Frans
AU - Schaart, G.
AU - Desmet, Valeer J.
PY - 1990
Y1 - 1990
N2 - Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.
AB - Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.
KW - cytokeratins
KW - hepatoblastoma
KW - histogenesis
KW - immunohistochemistry
UR - http://www.scopus.com/inward/record.url?scp=0025058655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025058655&partnerID=8YFLogxK
U2 - 10.1016/0046-8177(90)90231-S
DO - 10.1016/0046-8177(90)90231-S
M3 - Article
VL - 21
SP - 302
EP - 308
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 3
ER -