A cytokeratin-immunohistochemical study of hepatoblastoma

Peter Van Eyken, Raf Sciot, Francesco Callea, Frans Ramaekers, G. Schaart, Valeer J. Desmet

Research output: Contribution to journalArticle

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Abstract

Six cases of hepatoblastoma (five epithelial, one mixed epithelial-mesenchymal) were studied on serially cut cryostat sections, using a panel of monoclonal antibodies directed against individual cytokeratins, vimentin, and desmin, in an indirect immunoperoxidase procedure. Embryonic and fetal-type tumor cells expressed the "hepatocellular" cytokeratins no. 8 and 18 but, surprisingly, also expressed the "bile duct type" cytokeratin no. 19. In addition, two cases had a number of tumor cells which were also positive for the "bile duct type" cytokeratin no. 7. Cells embedded in osteoid-like material were immunoreactive for vimentin but also for cytokeratins no. 7, 18, and 19. Gel electrophoresis, and Western blotting of cytoskeletal extracts, confirmed the immunohistochemical data. The implications of these findings for the histogenesis of hepatoblastoma are discussed in this report.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalHuman Pathology
Volume21
Issue number3
DOIs
Publication statusPublished - 1990

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Hepatoblastoma
Keratins
Vimentin
Bile Ducts
Desmin
Electrophoresis
Neoplasms
Cell Count
Western Blotting
Gels
Monoclonal Antibodies

Keywords

  • cytokeratins
  • hepatoblastoma
  • histogenesis
  • immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Van Eyken, P., Sciot, R., Callea, F., Ramaekers, F., Schaart, G., & Desmet, V. J. (1990). A cytokeratin-immunohistochemical study of hepatoblastoma. Human Pathology, 21(3), 302-308. https://doi.org/10.1016/0046-8177(90)90231-S

A cytokeratin-immunohistochemical study of hepatoblastoma. / Van Eyken, Peter; Sciot, Raf; Callea, Francesco; Ramaekers, Frans; Schaart, G.; Desmet, Valeer J.

In: Human Pathology, Vol. 21, No. 3, 1990, p. 302-308.

Research output: Contribution to journalArticle

Van Eyken, P, Sciot, R, Callea, F, Ramaekers, F, Schaart, G & Desmet, VJ 1990, 'A cytokeratin-immunohistochemical study of hepatoblastoma', Human Pathology, vol. 21, no. 3, pp. 302-308. https://doi.org/10.1016/0046-8177(90)90231-S
Van Eyken, Peter ; Sciot, Raf ; Callea, Francesco ; Ramaekers, Frans ; Schaart, G. ; Desmet, Valeer J. / A cytokeratin-immunohistochemical study of hepatoblastoma. In: Human Pathology. 1990 ; Vol. 21, No. 3. pp. 302-308.
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