A defect in early myogenesis causes Otitis media in two mouse models of 22q11.2 deletion syndrome

Jennifer C. Fuchs, Jennifer F. Linden, Antonio Baldini, Abigail S. Tucker

Research output: Contribution to journalArticlepeer-review


Otitis media (OM), the inflammation of the middle ear, is the most common disease and cause for surgery in infants worldwide. Chronic Otitis media with effusion (OME) often leads to conductive hearing loss and is a common feature of a number of craniofacial syndromes, such as 22q11.2 Deletion Syndrome (22q11.2DS). OM is more common in children because the more horizontal position of the Eustachian tube (ET) in infants limits or delays clearance of middle ear effusions. Some mouse models with OM have shown alterations in the morphology and angle of the ET. Here, we present a novel mechanism in which OM is caused not by a defect in the ET itself but in the muscles that control its function. Our results showthat in two mouse models of 22q11.2DS (Df1/+ and Tbx1+/-) presenting with bi-or unilateral OME, the fourth pharyngeal arch-derived levator veli palatini muscles were hypoplastic, which was associated with an earlier altered pattern of MyoD expression. Importantly, in mice with unilateral OME, the side with the inflammation was associated with significantly smaller muscles than the contralateral unaffected ear. Functional tests examining ET patency confirmed a reduced clearing ability in the heterozygous mice. Our findings are also of clinical relevance as targeting hypoplastic muscles might present a novel preventative measure for reducing the high rates of OM in 22q11.2DS patients.

Original languageEnglish
Article numberddu604
Pages (from-to)1869-1882
Number of pages14
JournalHuman Molecular Genetics
Issue number7
Publication statusPublished - Oct 28 2014

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology


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