A definition of the Mycobacterium avium complex for taxonomical and clinical purposes, a review

J van Ingen, CY Turenne, E Tortoli, RJ Wallace, BA Brown-Elliott

Research output: Contribution to journalArticle

Abstract

Nontuberculous mycobacteria, particularly the Mycobacterium avium complex (MAC) bacteria, are increasingly recognized as opportunistic pathogens of humans. As a result, studies on antibiotic treatment and taxonomy of the MAC are intensifying, but an updated definition of what constitutes the MAC, either for taxonomical studies or for clinical purposes, is lacking. On the basis of literature review and phylogenetic analyses, we propose to define the MAC as a grouping of slow-growing mycobacteria that show corresponding values in at least two of the following targets against either M. avium ATCC 25291T or Mycobacterium intracellulare ATCC 13950T: >99.4 % sequence identity for the full 16S rRNA gene, >98.7 % for the partial (5') 16S rRNA gene, >97.3 % for hsp65 and >94.4 % for rpoB region V. A >97.5 % value in concatenated analyses of >2500 bp that includes 16S rRNA, hsp65 and rpoB gene sequence data or ≥85 % average nucleotide identity to M. avium ATCC 25291T or M. intracellulare ATCC 13950T on basis of whole genome sequencing data is recommended. This molecular definition is based on the distances observed between the classical members of the MAC, M. avium and M. intracellulare. Applying this definition, the complex currently consists of 12 validly published species: Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium chimaera, Mycobacterium colombiense, Mycobacterium arosiense, Mycobacterium vulneris, Mycobacterium bouchedurhonense, Mycobacterium timonense, Mycobacterium marseillense, Mycobacterium yongonense, Mycobacterium paraintracellulare and Mycobacterium lepraemurium.
Original languageEnglish
Pages (from-to)3666-3677
Number of pages12
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number11
DOIs
Publication statusPublished - 2018

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Mycobacterium avium complex
Mycobacterium avium Complex
Mycobacterium
gene
chimera
Mycobacterium intracellulare
literature review
Mycobacterium avium
antibiotics
genome
pathogen
phylogenetics
bacterium
ribosomal RNA
rRNA Genes
Mycobacterium lepraemurium
Nontuberculous Mycobacteria

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A definition of the Mycobacterium avium complex for taxonomical and clinical purposes, a review. / van Ingen, J; Turenne, CY; Tortoli, E; Wallace, RJ; Brown-Elliott, BA.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 11, 2018, p. 3666-3677.

Research output: Contribution to journalArticle

van Ingen, J ; Turenne, CY ; Tortoli, E ; Wallace, RJ ; Brown-Elliott, BA. / A definition of the Mycobacterium avium complex for taxonomical and clinical purposes, a review. In: International Journal of Systematic and Evolutionary Microbiology. 2018 ; Vol. 68, No. 11. pp. 3666-3677.
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abstract = "Nontuberculous mycobacteria, particularly the Mycobacterium avium complex (MAC) bacteria, are increasingly recognized as opportunistic pathogens of humans. As a result, studies on antibiotic treatment and taxonomy of the MAC are intensifying, but an updated definition of what constitutes the MAC, either for taxonomical studies or for clinical purposes, is lacking. On the basis of literature review and phylogenetic analyses, we propose to define the MAC as a grouping of slow-growing mycobacteria that show corresponding values in at least two of the following targets against either M. avium ATCC 25291T or Mycobacterium intracellulare ATCC 13950T: >99.4 {\%} sequence identity for the full 16S rRNA gene, >98.7 {\%} for the partial (5') 16S rRNA gene, >97.3 {\%} for hsp65 and >94.4 {\%} for rpoB region V. A >97.5 {\%} value in concatenated analyses of >2500 bp that includes 16S rRNA, hsp65 and rpoB gene sequence data or ≥85 {\%} average nucleotide identity to M. avium ATCC 25291T or M. intracellulare ATCC 13950T on basis of whole genome sequencing data is recommended. This molecular definition is based on the distances observed between the classical members of the MAC, M. avium and M. intracellulare. Applying this definition, the complex currently consists of 12 validly published species: Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium chimaera, Mycobacterium colombiense, Mycobacterium arosiense, Mycobacterium vulneris, Mycobacterium bouchedurhonense, Mycobacterium timonense, Mycobacterium marseillense, Mycobacterium yongonense, Mycobacterium paraintracellulare and Mycobacterium lepraemurium.",
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