A derivatization procedure suitable for HPLC analysis of clenbuterol

Pier Antonio Biondi, Luca Guidotti, Marco Montana, Francesca Manca, Gianfranco Brambilla, Claudio Lucarelli

Research output: Contribution to journalArticle


A precolumn derivatization method has been set up for the analysis of Clenbuterol by high-performance liquid chromatography (HPLC) with UV detection. After preliminary studies, N, N-dimethylaniline was chosen as reagent against Clenbuterol diazonium salt to form a diazocompound. The final derivative was separated on a reversed-phase column using a phosphate buffer-acetonitrile mixture as eluent. The reproducibility was increased using a compound homologous to Clenbuterol as the internal standard. The method was applied to the analysis of Clenbuterol in calfurine.

Original languageEnglish
Pages (from-to)190-193
Number of pages4
JournalJournal of Chromatographic Science
Issue number5
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Analytical Chemistry

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