A different microbiome gene repertoire in the airways of cystic fibrosis patients with severe lung disease

Giovanni Bacci, Alessio Mengoni, Ersilia Fiscarelli, Nicola Segata, Giovanni Taccetti, Daniela Dolce, Patrizia Paganin, Patrizia Morelli, Vanessa Tuccio, Alessandra De Alessandri, Vincenzina Lucidi, Annamaria Bevivino

Research output: Contribution to journalArticle

Abstract

In recent years, next-generation sequencing (NGS) was employed to decipher the structure and composition of the microbiota of the airways in cystic fibrosis (CF) patients. However, little is still known about the overall gene functions harbored by the resident microbial populations and which specific genes are associated with various stages of CF lung disease. In the present study, we aimed to identify the microbial gene repertoire of CF microbiota in twelve patients with severe and normal/mild lung disease by performing sputum shotgun metagenome sequencing. The abundance of metabolic pathways encoded by microbes inhabiting CF airways was reconstructed from the metagenome. We identified a set of metabolic pathways differently distributed in patients with different pulmonary function; namely, pathways related to bacterial chemotaxis and flagellar assembly, as well as genes encoding efflux-mediated antibiotic resistance mechanisms and virulence-related genes. The results indicated that the microbiome of CF patients with low pulmonary function is enriched in virulence-related genes and in genes encoding efflux-mediated antibiotic resistance mechanisms. Overall, the microbiome of severely affected adults with CF seems to encode different mechanisms for the facilitation of microbial colonization and persistence in the lung, consistent with the characteristics of multidrug-resistant microbial communities that are commonly observed in patients with severe lung disease.

Original languageEnglish
Article number1654
JournalInternational Journal of Molecular Sciences
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

cystic fibrosis
Pulmonary diseases
Microbiota
Cystic Fibrosis
genes
lungs
Lung Diseases
Genes
Metagenome
Gene encoding
pulmonary functions
Antibiotics
virulence
efflux
Microbial Drug Resistance
Metabolic Networks and Pathways
sequencing
antibiotics
Lung
Virulence

Keywords

  • Bioinformatics
  • Cystic fibrosis
  • Lung disease
  • Lung microbiome
  • Metabolic pathways
  • Shotgun metagenomics
  • Virulence genes

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

A different microbiome gene repertoire in the airways of cystic fibrosis patients with severe lung disease. / Bacci, Giovanni; Mengoni, Alessio; Fiscarelli, Ersilia; Segata, Nicola; Taccetti, Giovanni; Dolce, Daniela; Paganin, Patrizia; Morelli, Patrizia; Tuccio, Vanessa; De Alessandri, Alessandra; Lucidi, Vincenzina; Bevivino, Annamaria.

In: International Journal of Molecular Sciences, Vol. 18, No. 8, 1654, 01.08.2017.

Research output: Contribution to journalArticle

Bacci, G, Mengoni, A, Fiscarelli, E, Segata, N, Taccetti, G, Dolce, D, Paganin, P, Morelli, P, Tuccio, V, De Alessandri, A, Lucidi, V & Bevivino, A 2017, 'A different microbiome gene repertoire in the airways of cystic fibrosis patients with severe lung disease', International Journal of Molecular Sciences, vol. 18, no. 8, 1654. https://doi.org/10.3390/ijms18081654
Bacci, Giovanni ; Mengoni, Alessio ; Fiscarelli, Ersilia ; Segata, Nicola ; Taccetti, Giovanni ; Dolce, Daniela ; Paganin, Patrizia ; Morelli, Patrizia ; Tuccio, Vanessa ; De Alessandri, Alessandra ; Lucidi, Vincenzina ; Bevivino, Annamaria. / A different microbiome gene repertoire in the airways of cystic fibrosis patients with severe lung disease. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 8.
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