A dot immunobinding assay to detect anti-α-gliadin antibodies in celiac disease

Manuela Bittolo; Tarcisio Not; Sandra Perticarari; Sabina Cauci; Giorgio Graziosi; Roberto Marzari

A dot immunobinding assay to detect anti-α-gliadin antibodies in celiac disease

Manuela Bittolo, Tarcisio Not, Sandra Perticarari, Sabina Cauci, Giorgio Graziosi, Roberto Marzari

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.

Original language	English
Pages (from-to)	337-341
Number of pages	5
Journal	Journal of Pediatric Gastroenterology and Nutrition
Volume	11
Issue number	3
Publication status	Published - 1990

Keywords

Celiac disease
Dot immunobinding assay
Enzyme-linked immunosorbent assay
IgA and IgG antibodies
Nitrocellulose
α-Gliadin

ASJC Scopus subject areas

Gastroenterology
Pediatrics, Perinatology, and Child Health
Histology
Medicine (miscellaneous)
Food Science

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abstract = "A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.",

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T1 - A dot immunobinding assay to detect anti-α-gliadin antibodies in celiac disease

AU - Bittolo, Manuela

AU - Not, Tarcisio

AU - Perticarari, Sandra

AU - Cauci, Sabina

AU - Graziosi, Giorgio

AU - Marzari, Roberto

PY - 1990

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N2 - A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.

AB - A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.

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