A dot immunobinding assay to detect anti-α-gliadin antibodies in celiac disease

Manuela Bittolo, Tarcisio Not, Sandra Perticarari, Sabina Cauci, Giorgio Graziosi, Roberto Marzari

Research output: Contribution to journalArticlepeer-review

Abstract

A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.

Original languageEnglish
Pages (from-to)337-341
Number of pages5
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume11
Issue number3
Publication statusPublished - 1990

Keywords

  • Celiac disease
  • Dot immunobinding assay
  • Enzyme-linked immunosorbent assay
  • IgA and IgG antibodies
  • Nitrocellulose
  • α-Gliadin

ASJC Scopus subject areas

  • Gastroenterology
  • Pediatrics, Perinatology, and Child Health
  • Histology
  • Medicine (miscellaneous)
  • Food Science

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