Abstract
A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.
| Original language | English |
|---|---|
| Pages (from-to) | 337-341 |
| Number of pages | 5 |
| Journal | Journal of Pediatric Gastroenterology and Nutrition |
| Volume | 11 |
| Issue number | 3 |
| Publication status | Published - 1990 |
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Keywords
- Celiac disease
- Dot immunobinding assay
- Enzyme-linked immunosorbent assay
- IgA and IgG antibodies
- Nitrocellulose
- α-Gliadin
ASJC Scopus subject areas
- Gastroenterology
- Pediatrics, Perinatology, and Child Health
- Histology
- Medicine (miscellaneous)
- Food Science
Cite this
A dot immunobinding assay to detect anti-α-gliadin antibodies in celiac disease. / Bittolo, Manuela; Not, Tarcisio; Perticarari, Sandra; Cauci, Sabina; Graziosi, Giorgio; Marzari, Roberto.
In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 11, No. 3, 1990, p. 337-341.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A dot immunobinding assay to detect anti-α-gliadin antibodies in celiac disease
AU - Bittolo, Manuela
AU - Not, Tarcisio
AU - Perticarari, Sandra
AU - Cauci, Sabina
AU - Graziosi, Giorgio
AU - Marzari, Roberto
PY - 1990
Y1 - 1990
N2 - A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.
AB - A dot immunobinding assay to detect anti-α-gliadin-specific antibodies in the sera or whole blood of enteropathic patients is described here. The method is based on the adsorption of a-gliadin as a spot onto nitrocellulose sheets. After incubation with the patient sample, the detection of specific antibodies is performed with alkaline phosphatase-conjugated goat anti-human (IgA or IgG) antibodies. Twenty-one celiac serum samples to-gether with 18 enteropathic or disease controls and 44 healthy controls were analyzed. The classical ELISA test and the dot test gave comparable results. The dot test gave reliable result even when whole blood was tested. The method proved to be simple and sensitive.
KW - Celiac disease
KW - Dot immunobinding assay
KW - Enzyme-linked immunosorbent assay
KW - IgA and IgG antibodies
KW - Nitrocellulose
KW - α-Gliadin
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UR - http://www.scopus.com/inward/citedby.url?scp=0025081865&partnerID=8YFLogxK
M3 - Article
C2 - 2246715
AN - SCOPUS:0025081865
VL - 11
SP - 337
EP - 341
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - 3
ER -