A double determinant radioimmunoassay Mov2-MOv8 for monitoring ovarian carcinomas: definition of the methodology.

A. Diotti, O. Santoro, L. Mantovani, M. Regazzoni, R. Fontanelli, F. Ravagnani, S. Miotti, S. Ménard, M. I. Colnaghi

Research output: Contribution to journalArticle

Abstract

A double-determinant radioimmunoassay for the detection of circulating antigens associated with human ovarian carcinoma was developed using two monoclonal antibodies: MOv2 and MOv8 employed respectively as catcher and tracer. The development of the method through three different procedures enabled us to detect the presence of CaMOv2-CaMOv8 carrying molecules in 14 out of 15 ascitic fluids from ovarian carcinoma patients whose tumors were found to be positive with MOv2 and MOv8 monoclonal antibodies by immunofluorescence. Moreover, 13 out of 15 ovarian carcinoma patients presented high levels of antigen in their serum (60-170 Ua/ml). Low levels of antigen were observed in the normal population, the values ranging from 30-40 Ua/ml. However, in 13 out of 100 apparently healthy women high levels of antigen were found in the serum.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalInternational Journal of Biological Markers
Volume2
Issue number3
Publication statusPublished - Sep 1987

ASJC Scopus subject areas

  • Biochemistry
  • Immunology

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    Diotti, A., Santoro, O., Mantovani, L., Regazzoni, M., Fontanelli, R., Ravagnani, F., Miotti, S., Ménard, S., & Colnaghi, M. I. (1987). A double determinant radioimmunoassay Mov2-MOv8 for monitoring ovarian carcinomas: definition of the methodology. International Journal of Biological Markers, 2(3), 161-168.