A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor

E. Maestrini, L. Tamagnone, P. Longati, O. Cremona, M. Gulisano, S. Bione, F. Tamanini, B. G. Neel, D. Toniolo, P. M. Comoglio

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

In hunting for unknown genes on the human X chromosome, we identified a cDNA in Xq28 encoding a transmembrane protein (SEX) of 1871 amino acids. SEX shares significant homology with the extracellular domain of the receptors encoded by the oncogenes MET, RON, and SEA [hepatocyte growth factor (HGF) receptor family]. Further screenings of cDNA libraries identified three additional sequences closely related to SEX; these were named SEP, OCT, and NOV and were located on human chromosomes 3p, 1, and 3q, respectively. The proteins encoded by these genes contain large cytoplasmic domains characterized by a distinctive highly conserved sequence (SEX domain). Northern blot analysis revealed different expression of the SEX family of genes in fetal tissues, with SEX, OCT, and NOV predominantly expressed in brain, and SEP expressed at highest levels in kidney. In situ hybridization analysis revealed that SEX has a distinctive pattern of expression in the developing nervous system of the mouse, where it is found in postmitotic neurons from the first stages of neuronal differentiation (9.5 day postcoitus). The SEX protein (220 kDa) is glycosylated and exposed at the cell surface. Unlike the receptors of the HGF family, p220(SEX), a MET-SEX chimera or a constitutively dimerized TPR-SEX does not show tyrosine kinase activity. These data define a gene family (SEX family) involved in the development of neural and epithelial tissues, which encodes putative receptors with unexpected enzymatic or binding properties.

Original languageEnglish
Pages (from-to)674-678
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number2
DOIs
Publication statusPublished - Jan 23 1996

Fingerprint

Proto-Oncogene Proteins c-met
Chromosomes, Human, X
Neurologic Mutant Mice
Genes
Proteins
Conserved Sequence
Chromosomes, Human, Pair 1
Human Chromosomes
Gene Library
Oncogenes
Northern Blotting
Protein-Tyrosine Kinases
Nervous System
In Situ Hybridization
Fetus
Epithelium
Complementary DNA
Kidney
Neurons
Amino Acids

Keywords

  • MSP
  • neural development
  • RON
  • X chromosome

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. / Maestrini, E.; Tamagnone, L.; Longati, P.; Cremona, O.; Gulisano, M.; Bione, S.; Tamanini, F.; Neel, B. G.; Toniolo, D.; Comoglio, P. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 2, 23.01.1996, p. 674-678.

Research output: Contribution to journalArticle

Maestrini, E. ; Tamagnone, L. ; Longati, P. ; Cremona, O. ; Gulisano, M. ; Bione, S. ; Tamanini, F. ; Neel, B. G. ; Toniolo, D. ; Comoglio, P. M. / A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 2. pp. 674-678.
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AU - Gulisano, M.

AU - Bione, S.

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AU - Comoglio, P. M.

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