A fast and simple in situ-PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues

Tommaso Mancuso, Sergio Crovella, Stefania Casazza, Maria Pia Pescarolo, Luana Clerico, Patrizia Russo, Silvio Parodi

Research output: Contribution to journalArticle

Abstract

We describe a simple and fast method for the detection and localization of low copy numbers of HPV DNA in formalin-fixed and paraffin-embedded archival tissues. We have developed a protocol for direct in situ-PCR in order to demonstrate its convenience in rapid and reproducible assessment of HPV infection in unknown biopsies. The morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected only in virus target cells.

Original languageEnglish
Pages (from-to)527-531
Number of pages5
JournalInternational Journal of Oncology
Volume11
Issue number3
Publication statusPublished - 1997

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Keywords

  • Archival samples
  • Human papilloma virus
  • In situ hybridization
  • In situ-PCR
  • Vital infection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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