Brza metoda za genotipiziranje DEFB1 - 44C/G SNP-a kod brazilskih pacijenata s parodontitisom

Renata Cimões; Rafael Rafael Amorim Cavalcanti de Siqueira; Sergio Crovella; Paulo Roberto Eleutério de Souza; Nikos Donos

doi:10.15644/asc48/3/5

Brza metoda za genotipiziranje DEFB1 - 44C/G SNP-a kod brazilskih pacijenata s parodontitisom

Translated title of the contribution: A fast method for DEFB1-44C/G snp genotyping in brazilian patients with periodontitis

Renata Cimões, Rafael Rafael Amorim Cavalcanti de Siqueira, Sergio Crovella, Paulo Roberto Eleutério de Souza, Nikos Donos

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article

Abstract

Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparisonto healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigatethe role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

Translated title of the contribution	A fast method for DEFB1-44C/G snp genotyping in brazilian patients with periodontitis
Original language	Undefined/Unknown
Pages (from-to)	208-215
Number of pages	8
Journal	Acta Stomatologica Croatica
Volume	48
Issue number	3
DOIs	https://doi.org/10.15644/asc48/3/5
Publication status	Published - 2014

Keywords

Antimicrobial cationic peptides
DEFB1 protein
Defensins
Diabetes complications
Epithelial cells
Human
Periodontitis

ASJC Scopus subject areas

Dentistry(all)

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Cimões, R., de Siqueira, R. R. A. C., Crovella, S., de Souza, P. R. E., & Donos, N. (2014). Brza metoda za genotipiziranje DEFB1 - 44C/G SNP-a kod brazilskih pacijenata s parodontitisom. Acta Stomatologica Croatica, 48(3), 208-215. https://doi.org/10.15644/asc48/3/5

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abstract = "Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparisonto healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigatethe role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.",

keywords = "Antimicrobial cationic peptides, DEFB1 protein, Defensins, Diabetes complications, Epithelial cells, Human, Periodontitis",

author = "Renata Cim{\~o}es and {de Siqueira}, {Rafael Rafael Amorim Cavalcanti} and Sergio Crovella and {de Souza}, {Paulo Roberto Eleut{\'e}rio} and Nikos Donos",

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AU - Cimões, Renata

AU - de Siqueira, Rafael Rafael Amorim Cavalcanti

AU - Crovella, Sergio

AU - de Souza, Paulo Roberto Eleutério

AU - Donos, Nikos

PY - 2014

Y1 - 2014

N2 - Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparisonto healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigatethe role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

AB - Aim: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparisonto healthy controls. Material and Methods: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients. Results: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently. Conclusion: Further studies are necessary in order to investigatethe role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

KW - Antimicrobial cationic peptides

KW - DEFB1 protein

KW - Defensins

KW - Diabetes complications

KW - Epithelial cells

KW - Human

KW - Periodontitis

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