A fast method for the detection of irinotecan in plasma samples by combining solid phase extraction and differential pulse voltammetry

Gregorio Bonazza, Stefano Tartaggia, Giuseppe Toffoli, Federico Polo, Salvatore Daniele

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, a fast method for the detection of irinotecan (CPT-11) in plasma samples was investigated. CPT-11 is widely used in a number of chemotherapeutic treatments of several solid tumors. The method is based on the combination of a solid phase extraction and an electrochemical detection step. The extraction of CPT-11 from plasma was performed using solid phase extraction (SPE) columns and acetonitrile as eluent. The procedure included also a cleaning step to eliminate interference due to plasma endogenous compounds and the co-therapeutics 5-fluoroacil (5-FU) and folinic acid (FA). The latter are administered together with CPT-11 in the FOLFIRI regimen. The detection of CPT-11 was performed by differential pulse voltammetry at a glassy carbon electrode (GCE) in basified acetonitrile media. Under these conditions, a well-defined peak due to the oxidation of the tertiary ammine end of CPT-11, also free from interference due to main metabolites, was obtained. Calibration plots showed a good linear response with limit of detection and quantification of 1.10 × 10−7 and 3.74 × 10−7 M, respectively. The suitability of the method proposed here for clinical applications was verified by determining the concentration of CPT-11 in plasma samples of an oncological patient, collected after 30 and 180 min from the infusion of the drug. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1585-1595
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume412
Issue number7
DOIs
Publication statusPublished - Mar 1 2020

Keywords

  • FOLFIRI regimen
  • Irinotecan
  • Solid phase extraction
  • Voltammetric detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Fingerprint Dive into the research topics of 'A fast method for the detection of irinotecan in plasma samples by combining solid phase extraction and differential pulse voltammetry'. Together they form a unique fingerprint.

Cite this