A fluorescent one-dimensional photonic crystal for label-free biosensing based on bloch surface waves

Francesca Frascella, Serena Ricciardi, Paola Rivolo, Valeria Moi, Fabrizio Giorgis, Emiliano Descrovi, Francesco Michelotti, Peter Munzert, Norbert Danz, Lucia Napione, Maria Alvaro, Federico Bussolino

Research output: Contribution to journalArticlepeer-review

Abstract

A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.

Original languageEnglish
Pages (from-to)2011-2022
Number of pages12
JournalSensors (Switzerland)
Volume13
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • Bragg mirrors
  • Fluorescence
  • Label-free sensing
  • Multilayers
  • Surface electromagnetic waves

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

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