A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Maria R. Rios; Gloria Garoffolo; Giulia Rinaldi; Alicia Megia-Fernandez; Silvia Ferrari; Calum T. Robb; Adriano G. Rossi; Maurizio Pesce; Mark Bradley

doi:10.1039/d0cc07028a

A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Maria R. Rios, Gloria Garoffolo, Giulia Rinaldi, Alicia Megia-Fernandez, Silvia Ferrari, Calum T. Robb, Adriano G. Rossi, Maurizio Pesce, Mark Bradley

Centro Cardiologico Monzino

Research output: Contribution to journal › Article › peer-review

Abstract

A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils. This journal is

Original language	English
Pages (from-to)	97-100
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	1
DOIs	https://doi.org/10.1039/d0cc07028a
Publication status	Published - Jan 4 2021

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d0cc07028a

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A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation. / Rios, Maria R.; Garoffolo, Gloria; Rinaldi, Giulia; Megia-Fernandez, Alicia; Ferrari, Silvia; Robb, Calum T.; Rossi, Adriano G.; Pesce, Maurizio; Bradley, Mark.

In: Chemical Communications, Vol. 57, No. 1, 04.01.2021, p. 97-100.

Research output: Contribution to journal › Article › peer-review

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AU - Ferrari, Silvia

AU - Robb, Calum T.

AU - Rossi, Adriano G.

AU - Pesce, Maurizio

AU - Bradley, Mark

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A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Abstract

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