A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation

Maria R. Rios, Gloria Garoffolo, Giulia Rinaldi, Alicia Megia-Fernandez, Silvia Ferrari, Calum T. Robb, Adriano G. Rossi, Maurizio Pesce, Mark Bradley

Research output: Contribution to journalArticlepeer-review

Abstract

A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils. This journal is

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalChemical Communications
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 4 2021

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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