A forskolin and verapamil sensitive K+ current in human tracheal cells

Luis J V Galietta, Andrea Rasola, Virginia Barone, Dieter C. Gruenert, Giovanni Romeo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A voltage-dependent K+ current has been revealed in whole-cell recordings carried out on immortalized cells obtained from the human tracheal epithelium. At positive membrane potentials the current shows a time dependent inactivation which is accelerated by increasing the depolarizing step. Forskolin, a direct activator of adenylyl cyclase, and verapamil, a Ca2+ channel blocker, induce the K+ current to inactivate more rapidly. Control experiments show that the action of these two compounds is not mediated by cyclic AMP and Ca2+. The application of 1,9-dideoxyforskolin, an analogue which does not stimulate adenylate cyclase, inhibits the current in the same way as forskolin; on the contrary, the dibutyryl analogue of cyclic AMP is ineffective. Furthermore, eliminating extracellular Ca2+ does not affect K+ current kinetics. Tetraethylammonium is an effective blocker of this current with an IC50 of 0.3 mM.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number3
DOIs
Publication statusPublished - Sep 30 1991

Fingerprint

Colforsin
Verapamil
Adenylyl Cyclases
Cyclic AMP
Bucladesine
Tetraethylammonium
Patch-Clamp Techniques
Membrane Potentials
Inhibitory Concentration 50
Epithelium
Membranes
Kinetics
Electric potential
Experiments
1,9-dideoxyforskolin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A forskolin and verapamil sensitive K+ current in human tracheal cells. / Galietta, Luis J V; Rasola, Andrea; Barone, Virginia; Gruenert, Dieter C.; Romeo, Giovanni.

In: Biochemical and Biophysical Research Communications, Vol. 179, No. 3, 30.09.1991, p. 1155-1160.

Research output: Contribution to journalArticle

Galietta, Luis J V ; Rasola, Andrea ; Barone, Virginia ; Gruenert, Dieter C. ; Romeo, Giovanni. / A forskolin and verapamil sensitive K+ current in human tracheal cells. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 179, No. 3. pp. 1155-1160.
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