A forskolin and verapamil sensitive K+ current in human tracheal cells

Luis J V Galietta, Andrea Rasola, Virginia Barone, Dieter C. Gruenert, Giovanni Romeo

Research output: Contribution to journalArticle

Abstract

A voltage-dependent K+ current has been revealed in whole-cell recordings carried out on immortalized cells obtained from the human tracheal epithelium. At positive membrane potentials the current shows a time dependent inactivation which is accelerated by increasing the depolarizing step. Forskolin, a direct activator of adenylyl cyclase, and verapamil, a Ca2+ channel blocker, induce the K+ current to inactivate more rapidly. Control experiments show that the action of these two compounds is not mediated by cyclic AMP and Ca2+. The application of 1,9-dideoxyforskolin, an analogue which does not stimulate adenylate cyclase, inhibits the current in the same way as forskolin; on the contrary, the dibutyryl analogue of cyclic AMP is ineffective. Furthermore, eliminating extracellular Ca2+ does not affect K+ current kinetics. Tetraethylammonium is an effective blocker of this current with an IC50 of 0.3 mM.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number3
DOIs
Publication statusPublished - Sep 30 1991

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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