Abstract
An unbalance between Abs that recognize an autoantigen (idiotypes; IDs) and Igs that bind such Abs (anti-IDs) is considered a functional event in autoimmune disorders. We investigated the presence of an ID/anti-ID network in celiac disease (CD), a condition in which antitissue transglutaminase 2 (TG2) Abs are suspected to contribute to CD pathogenesis. To characterize the ID side, we reproduced by in vitro yeast display the intestine-resident Abs from CD and control patients. These TG2-specific IDs were used to identify potential anti-IDs in the serum. We observed elevated titers of anti-IDs in asymptomatic patients with predisposition to CD and demonstrated that anti-ID depletion from the serum restores a detectable humoral response against TG2. Our study provides an alternative approach to quantify CD-related autoantibodies in cases that would be defined "negative serology" with current diagnostic applications. Therefore, we suggest that developments of this technology could be designed for perspective routine tests.
Original language | English |
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Pages (from-to) | 1079-1087 |
Number of pages | 9 |
Journal | Journal of immunology (Baltimore, Md. : 1950) |
Volume | 202 |
Issue number | 4 |
DOIs | |
Publication status | Published - Feb 15 2019 |
Keywords
- Adolescent
- Adult
- Antibodies, Anti-Idiotypic/immunology
- Autoantibodies/blood
- Celiac Disease/genetics
- Child
- Child, Preschool
- Female
- GTP-Binding Proteins/immunology
- Glutens/genetics
- Humans
- Immunoglobulin Idiotypes/immunology
- Intestinal Mucosa/immunology
- Intestines/immunology
- Male
- Middle Aged
- Transglutaminases/immunology
- Young Adult