A functional role for some Fugu introns larger than the typical short ones: The example of the gene coding for ribosomal protein S7 and snoRNA U17

Francesco Cecconi, Claudia Crosio, Paolo Mariottini, Gianni Cesareni, Marcello Giorgi, Sydney Brenner, Francesco Amaldi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The compact genome of Fugu rubripes, with its very small introns, appears to be particularly suitable to study intron-encoded functions. We have analyzed the Fugu gene for ribosomal protein S7 (formerly S8, see Note), whose Xenopus homolog contains in its introns the coding sequences for the small nucleolar RNA U17. Except for intron length, the organization of the Fugu S7 gene is very similar to that of the Xenopus counterpart. The total length of the Fugu S7 gene is 3930 bp, compared with 12691 bp for Xenopus. This length difference is uniquely due to smaller introns. Although short, the six introns are longer than the ~100 bp size of most Fugu introns, as they host U17 RNA coding sequences. While four of the six U17 sequences are 'canonical', the remaining two represent diverged U17 pseudocopies. In fact, microinjection in Xenopus oocytes of in vitro synthesized Fugu transcripts containing the 'canonical' U17f sequence results in efficient production of mature U17 RNA, while injection of a transcript containing the U17ψb sequence does not.

Original languageEnglish
Pages (from-to)3167-3172
Number of pages6
JournalNucleic Acids Research
Volume24
Issue number16
Publication statusPublished - 1996

Fingerprint

Takifugu
Small Nucleolar RNA
Introns
Xenopus
Genes
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
Microinjections
ribosomal protein S7
Oocytes
Genome
RNA
Injections

ASJC Scopus subject areas

  • Genetics

Cite this

A functional role for some Fugu introns larger than the typical short ones : The example of the gene coding for ribosomal protein S7 and snoRNA U17. / Cecconi, Francesco; Crosio, Claudia; Mariottini, Paolo; Cesareni, Gianni; Giorgi, Marcello; Brenner, Sydney; Amaldi, Francesco.

In: Nucleic Acids Research, Vol. 24, No. 16, 1996, p. 3167-3172.

Research output: Contribution to journalArticle

Cecconi, Francesco ; Crosio, Claudia ; Mariottini, Paolo ; Cesareni, Gianni ; Giorgi, Marcello ; Brenner, Sydney ; Amaldi, Francesco. / A functional role for some Fugu introns larger than the typical short ones : The example of the gene coding for ribosomal protein S7 and snoRNA U17. In: Nucleic Acids Research. 1996 ; Vol. 24, No. 16. pp. 3167-3172.
@article{cd74921b4b3f4e2d8906870c80d31b8d,
title = "A functional role for some Fugu introns larger than the typical short ones: The example of the gene coding for ribosomal protein S7 and snoRNA U17",
abstract = "The compact genome of Fugu rubripes, with its very small introns, appears to be particularly suitable to study intron-encoded functions. We have analyzed the Fugu gene for ribosomal protein S7 (formerly S8, see Note), whose Xenopus homolog contains in its introns the coding sequences for the small nucleolar RNA U17. Except for intron length, the organization of the Fugu S7 gene is very similar to that of the Xenopus counterpart. The total length of the Fugu S7 gene is 3930 bp, compared with 12691 bp for Xenopus. This length difference is uniquely due to smaller introns. Although short, the six introns are longer than the ~100 bp size of most Fugu introns, as they host U17 RNA coding sequences. While four of the six U17 sequences are 'canonical', the remaining two represent diverged U17 pseudocopies. In fact, microinjection in Xenopus oocytes of in vitro synthesized Fugu transcripts containing the 'canonical' U17f sequence results in efficient production of mature U17 RNA, while injection of a transcript containing the U17ψb sequence does not.",
author = "Francesco Cecconi and Claudia Crosio and Paolo Mariottini and Gianni Cesareni and Marcello Giorgi and Sydney Brenner and Francesco Amaldi",
year = "1996",
language = "English",
volume = "24",
pages = "3167--3172",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = ". This work is written by (a) US Government employee(s) and is in the public domain in the US",
number = "16",

}

TY - JOUR

T1 - A functional role for some Fugu introns larger than the typical short ones

T2 - The example of the gene coding for ribosomal protein S7 and snoRNA U17

AU - Cecconi, Francesco

AU - Crosio, Claudia

AU - Mariottini, Paolo

AU - Cesareni, Gianni

AU - Giorgi, Marcello

AU - Brenner, Sydney

AU - Amaldi, Francesco

PY - 1996

Y1 - 1996

N2 - The compact genome of Fugu rubripes, with its very small introns, appears to be particularly suitable to study intron-encoded functions. We have analyzed the Fugu gene for ribosomal protein S7 (formerly S8, see Note), whose Xenopus homolog contains in its introns the coding sequences for the small nucleolar RNA U17. Except for intron length, the organization of the Fugu S7 gene is very similar to that of the Xenopus counterpart. The total length of the Fugu S7 gene is 3930 bp, compared with 12691 bp for Xenopus. This length difference is uniquely due to smaller introns. Although short, the six introns are longer than the ~100 bp size of most Fugu introns, as they host U17 RNA coding sequences. While four of the six U17 sequences are 'canonical', the remaining two represent diverged U17 pseudocopies. In fact, microinjection in Xenopus oocytes of in vitro synthesized Fugu transcripts containing the 'canonical' U17f sequence results in efficient production of mature U17 RNA, while injection of a transcript containing the U17ψb sequence does not.

AB - The compact genome of Fugu rubripes, with its very small introns, appears to be particularly suitable to study intron-encoded functions. We have analyzed the Fugu gene for ribosomal protein S7 (formerly S8, see Note), whose Xenopus homolog contains in its introns the coding sequences for the small nucleolar RNA U17. Except for intron length, the organization of the Fugu S7 gene is very similar to that of the Xenopus counterpart. The total length of the Fugu S7 gene is 3930 bp, compared with 12691 bp for Xenopus. This length difference is uniquely due to smaller introns. Although short, the six introns are longer than the ~100 bp size of most Fugu introns, as they host U17 RNA coding sequences. While four of the six U17 sequences are 'canonical', the remaining two represent diverged U17 pseudocopies. In fact, microinjection in Xenopus oocytes of in vitro synthesized Fugu transcripts containing the 'canonical' U17f sequence results in efficient production of mature U17 RNA, while injection of a transcript containing the U17ψb sequence does not.

UR - http://www.scopus.com/inward/record.url?scp=0029761129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029761129&partnerID=8YFLogxK

M3 - Article

C2 - 8774896

AN - SCOPUS:0029761129

VL - 24

SP - 3167

EP - 3172

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 16

ER -