A G-CSF functionalized PLLA scaffold for wound repair: An in vitro preliminary study

Cristiano Spadaccio, Alberto Rainer, Stefano De Porcellinis, Matteo Centola, Federico De Marco, Massimo Chello, Marcella Trombetta, Jorge A. Genovese

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Citations (Scopus)

Abstract

Targeting wound repair, we developed an electrospun poly-L-lactide fibrous scaffold functionalized with G-CSF, a growth factor which is widely recognized as important in wound healing homeostasis. The scaffold was characterized in terms of morphology, mechanical properties and in vitro capacity to induce organization of co-cultures of murine fibroblasts and keratinocytes into a dermo-epidermal multilayered structure. Our findings are consistent with the promotion of a nonhostile environment, in which seeded cells could arrange themselves in an appropriate topographic distribution of elements at different levels of maturation up to a cornified epithelium on the top layer, resembling native skin.

Original languageEnglish
Title of host publication2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
Pages843-846
Number of pages4
DOIs
Publication statusPublished - 2010
Event2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10 - Buenos Aires, Argentina
Duration: Aug 31 2010Sep 4 2010

Other

Other2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
CountryArgentina
CityBuenos Aires
Period8/31/109/4/10

Fingerprint

Scaffolds
Repair
Fibroblasts
Cell culture
Skin
Mechanical properties
Intercellular Signaling Peptides and Proteins
Keratinocytes

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Spadaccio, C., Rainer, A., De Porcellinis, S., Centola, M., De Marco, F., Chello, M., ... Genovese, J. A. (2010). A G-CSF functionalized PLLA scaffold for wound repair: An in vitro preliminary study. In 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10 (pp. 843-846). [5626796] https://doi.org/10.1109/IEMBS.2010.5626796

A G-CSF functionalized PLLA scaffold for wound repair : An in vitro preliminary study. / Spadaccio, Cristiano; Rainer, Alberto; De Porcellinis, Stefano; Centola, Matteo; De Marco, Federico; Chello, Massimo; Trombetta, Marcella; Genovese, Jorge A.

2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10. 2010. p. 843-846 5626796.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Spadaccio, C, Rainer, A, De Porcellinis, S, Centola, M, De Marco, F, Chello, M, Trombetta, M & Genovese, JA 2010, A G-CSF functionalized PLLA scaffold for wound repair: An in vitro preliminary study. in 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10., 5626796, pp. 843-846, 2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10, Buenos Aires, Argentina, 8/31/10. https://doi.org/10.1109/IEMBS.2010.5626796
Spadaccio C, Rainer A, De Porcellinis S, Centola M, De Marco F, Chello M et al. A G-CSF functionalized PLLA scaffold for wound repair: An in vitro preliminary study. In 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10. 2010. p. 843-846. 5626796 https://doi.org/10.1109/IEMBS.2010.5626796
Spadaccio, Cristiano ; Rainer, Alberto ; De Porcellinis, Stefano ; Centola, Matteo ; De Marco, Federico ; Chello, Massimo ; Trombetta, Marcella ; Genovese, Jorge A. / A G-CSF functionalized PLLA scaffold for wound repair : An in vitro preliminary study. 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10. 2010. pp. 843-846
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