A gas chromatographic method for the determination of di- and polyamines in human urine

S. Beninati, M. Piacentini, M. P. Argento Ceru

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.

Original languageEnglish
Pages (from-to)156-167
Number of pages12
JournalItalian Journal of Biochemistry
Volume27
Issue number3
Publication statusPublished - 1978

Fingerprint

1,6-diaminohexane
Diamines
Polyamines
Gases
Urine
Cadaverine
Ephedrine
Putrescine
Spermidine
Spermine
Amines
Detectors
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

A gas chromatographic method for the determination of di- and polyamines in human urine. / Beninati, S.; Piacentini, M.; Argento Ceru, M. P.

In: Italian Journal of Biochemistry, Vol. 27, No. 3, 1978, p. 156-167.

Research output: Contribution to journalArticle

@article{fc4f7349da674ed980d2a511045753bb,
title = "A gas chromatographic method for the determination of di- and polyamines in human urine",
abstract = "A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.",
author = "S. Beninati and M. Piacentini and {Argento Ceru}, {M. P.}",
year = "1978",
language = "English",
volume = "27",
pages = "156--167",
journal = "Italian Journal of Biochemistry",
issn = "0021-2938",
publisher = "Biomedia srl",
number = "3",

}

TY - JOUR

T1 - A gas chromatographic method for the determination of di- and polyamines in human urine

AU - Beninati, S.

AU - Piacentini, M.

AU - Argento Ceru, M. P.

PY - 1978

Y1 - 1978

N2 - A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.

AB - A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.

UR - http://www.scopus.com/inward/record.url?scp=0018145880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018145880&partnerID=8YFLogxK

M3 - Article

C2 - 738858

AN - SCOPUS:0018145880

VL - 27

SP - 156

EP - 167

JO - Italian Journal of Biochemistry

JF - Italian Journal of Biochemistry

SN - 0021-2938

IS - 3

ER -