A gas chromatographic method for the determination of di- and polyamines in human urine

S. Beninati, M. Piacentini, M. P. Argento Ceru

Research output: Contribution to journalArticle

Abstract

A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.

Original languageEnglish
Pages (from-to)156-167
Number of pages12
JournalItalian Journal of Biochemistry
Volume27
Issue number3
Publication statusPublished - 1978

ASJC Scopus subject areas

  • Biochemistry

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