We describe here a new procedure for analysing eleven sterols in environmental samples using gas chromatography-mass spectrometry (GC-MS). This consists of complete silylation of the sterols using N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with iodo-trimethylsilane (TMSI) and a base as catalyst. To assess the applicability of the method to environmental samples and to estimate the extraction efficiency we added increasing amounts of sterols to the same sediment sample and quantified them by the internal standard method. We also analyzed, in three replications, a sediment sample from the Adriatic Sea in order to establish the precision of the method.
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Earth and Planetary Sciences(all)