A GC-MS method for the analysis of fecal and plant sterols in sediment samples

Emilio Benfenati, Etienne Cools, Elena Fattore, Roberto Fanelli

Research output: Contribution to journalArticle

Abstract

We describe here a new procedure for analysing eleven sterols in environmental samples using gas chromatography-mass spectrometry (GC-MS). This consists of complete silylation of the sterols using N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with iodo-trimethylsilane (TMSI) and a base as catalyst. To assess the applicability of the method to environmental samples and to estimate the extraction efficiency we added increasing amounts of sterols to the same sediment sample and quantified them by the internal standard method. We also analyzed, in three replications, a sediment sample from the Adriatic Sea in order to establish the precision of the method.

Original languageEnglish
Pages (from-to)1393-1405
Number of pages13
JournalChemosphere
Volume29
Issue number7
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Earth and Planetary Sciences(all)

Fingerprint Dive into the research topics of 'A GC-MS method for the analysis of fecal and plant sterols in sediment samples'. Together they form a unique fingerprint.

  • Cite this