A genetic analysis of the adenine phosphoribosyl transferase locus in Chinese hamster V79-AP4 cells: relevance to mutagenesis studies

Carlo M. Colella, Silvana Simi, Theo Van Boxel, Daniela Talarico, Giuliano Della Valle, M. Luisa Carrozza, Duccio Fratta, Tullio Mariani, Antonio Piras, Marcella Simili, Giuseppe Rainaldi

Research output: Contribution to journalArticle

Abstract

The chromosomal location of the autosomal locus aprt has been investigated in the permanent Chinese hamster cell line V79-AP4 by standard somatic cell genetics methodologies. Aprt is functionally dizygous in V79-AP4 and the a alleles map on 2 chromosome 3 homologs, in agreement with the chromosome assignment of the gene in Chinese hamster primary cells. Chromosome G-banding and a Southern blot analysis of V79-AP4 DNA, using as a probe the cloned Chinese hamster aprt gene, have not revealed any structural alteration at either of the 2 aprt alleles. One of the chromosomes 3 has, however, a terminal deletion in its long arm and is therefore morphologically marked. These findings could make V79-AP4 an interesting cell system for the study of mutational mechanisms at the aprt locus in Chinese hamster.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume202
Issue number1
DOIs
Publication statusPublished - 1988

Keywords

  • Adenine phosphoribosyl transferase locus
  • Chinese hamster V79-AP4 cell line

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

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