A genomic, transcriptomic and proteomic look at the GE2270 producer Planobispora rosea, an uncommon actinomycete

Arianna Tocchetti, Roberta Bordoni, Giuseppe Gallo, Luca Petiti, Giorgio Corti, Silke Alt, Joao C S Cruz, Anna Maria Salzano, Andrea Scaloni, Anna Maria Puglia, Gianluca De Bellis, Clelia Peano, Stefano Donadio, Margherita Sosio

Research output: Contribution to journalArticlepeer-review

Abstract

We report the genome sequence of Planobispora rosea ATCC 53733, a mycelium-forming soil-dweller belonging to one of the lesser studied genera of Actinobacteria and producing the thiopeptide GE2270. The P. rosea genome presents considerable convergence in gene organization and function with other members in the family Streptosporangiaceae, with a significant number (44%) of shared orthologs. Patterns of gene expression in P. rosea cultures during exponential and stationary phase have been analyzed using whole transcriptome shotgun sequencing and by proteome analysis. Among the differentially abundant proteins, those involved in protein metabolism are particularly represented, including the GE2270-insensitive EF-Tu. Two proteins from the pbt cluster, directing GE2270 biosynthesis, slightly increase their abundance values over time. While GE2270 production starts during the exponential phase, most pbt genes, as analyzed by qRT-PCR, are down-regulated. The exception is represented by pbtA, encoding the precursor peptide of the ribosomally synthesized GE2270, whose expression reached the highest level at the entry into stationary phase.

Original languageEnglish
Article numbere0133705
JournalPLoS One
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 24 2015

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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